Product Name:
Nek6-pS206
Product Number:
ab-pk734
Target Full Name: NIMA (never-in-mitosis)-related protein-serine kinase 6
Target Alias: Kinase Nek6; NIMA; NIMA (never in mitosis gene a)-related expressed kinase 6; NIMA (never in mitosis gene a)-related kinase 6; NIMA-related kinase 6; NIMA-related serine/threonine kinase; Putative serine-threonine protein kinase; SID6-1512
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK734
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S206
Protein UniProt: Q9HC98
Protein SigNET: Q9HC98
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Nek6 sequence peptide Cat. No.: PE-04AOE99
Antibody Immunogen Sequence: AAH(pS)LVG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues A203 to G209; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues A203 to G209; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 36-38 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: Almost no significant cross-reactivities detected in HeLa cells.
Related Product 1: Nek6-pS206 blocking peptide
Scientific Background: Nek6 is a protein-serine/threonine kinase that is a member of the Other group of protein kinases in the Nek family. This kinase is highly expressed and widely distributed in most tested human tissues except apparently in the brain and spinal cord. Nek6 is closely related in its catalytic domain to the protein-serine/threonine kinase NimA of Aspergillus nidulans. Nek6 is phosphorylated and activated during M phase of the cell cycle, and its inhibition facilitates apoptosis. Inhibition of Nek6 function by either overexpression of an inactive Nek6 mutant or elimination of endogenous Nek6 by siRNA arrests cells in M phase and triggers apoptosis. Nek6 may be an oncoprotein (OP). Nek6 may contribute to tumourigenesis by suppressing p53/TP53-induced cancer cell senescence. Nek6 inhibition sensitizes human cancer cells towards premature senescence.
