Product Name:
Nek6-pS206
Product Number:
ab-pk734
Target Full Name: NIMA (never-in-mitosis)-related protein-serine kinase 6
Target Alias: Kinase Nek6; NIMA; NIMA (never in mitosis gene a)-related expressed kinase 6; NIMA (never in mitosis gene a)-related kinase 6; NIMA-related kinase 6; NIMA-related serine/threonine kinase; Putative serine-threonine protein kinase; SID6-1512
Product Type Specific: Nek6 protein kinase phosphosite-specific antibody
Antibody Code: PK734
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S206
Protein UniProt: Q9HC98
Protein SigNET: Nek6
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Nek6 sequence peptide Cat. No.: PE-04AOE99
Antibody Immunogen Sequence: AAH(pS)LVG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues A203 to G209. S206 phosphorylation stimulates phosphotransferase activity. This phosphosite is located in the kinase activation loop between catalytic subdomains VII and VIII. This is the major in vivo phosphorylation site in Nek6 (≥60 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). Nek6 is known to be phosphorylated at this site in vitro by NEK9.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Mouse | Platypus | Chicken | Nematode worm | Sea urchin.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 36-38 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: Almost no significant cross-reactivities detected in HeLa cells.
Related Product 1: Nek6 (203-209) pS206 antibody blocking peptide (Cat. No.: PE-04AOE99)
Related Product 2: Nek2 S171 phosphorylation antibody (Cat. No.: AB-PK732)
Related Product 3: Nek2 T170+S171 phosphorylation antibody (Cat. No.: AB-PK733)
Related Product 4: Nek7 T191+S195 phosphorylation antibody (Cat. No.: AB-PK735)
Related Product 5: Nek1 S1052 phosphorylation antibody (Cat. No.: AB-PK940)
Scientific Background: Nek6 is a protein-serine/threonine kinase that is a member of the Other group of protein kinases in the Nek family. It is important for mitotic cell cycle progression, particularly in regulating chromosome segregation, robust spindle formation, and the metaphase-anaphase transition. It is a globular, monomeric protein that features a NIMA-like catalytic domain and a flexible N-terminal domain that mediates protein interactions. Nek6 is closely related in its catalytic domain to the protein-serine/threonine kinase NimA of Aspergillus nidulans. It is activated by Nek9, and phosphorylation by checkpoint kinases Chk1/Chk2. It phosphorylates proteins such as Eg5 (kinesin) for control of spindle assembly, as well as STAT3, Histone H3, and others. Nek6 is highly expressed and widely distributed in most tested human tissues except apparently in the brain and spinal cord. Nek6 is phosphorylated and activated during M phase of the cell cycle, and its inhibition facilitates apoptosis. Inhibition of Nek6 function, by either overexpression of an inactive Nek6 mutant or elimination of endogenous Nek6 by siRNA, arrests cells in M phase and triggers apoptosis. Nek6 may be an oncoprotein (OP). Nek6 may contribute to tumorigenesis by suppressing p53/TP53-induced cancer cell senescence. It is often overexpressed in cancer cells (breast, liver, prostate, etc.), where it aids tumour progression by suppressing p53-induced cellular senescence. Nek6 inhibition sensitizes human cancer cells towards premature senescence. This description may include information annotated by UniProt and/or Google AI.