Product Name:
Nek2-pT170+pS171
Product Number:
ab-pk733
Target Full Name: NIMA (never-in-mitosis)-related protein-serine kinase 2
Target Alias: HSPK 21; Kinase Nek2; Nek2A; NIMA (never in mitosis gene a)-related kinase 2; NimA-like protein kinase 1; NimA-related protein kinase 2; NLK1
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK733
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T170+S171
Protein UniProt: P51955
Protein SigNET: P51955
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Nek2 sequence peptide Cat. No.: PE-04ABO90
Antibody Immunogen Sequence: HD(pT)(pS)FAK(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues H168 to K174; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues H168 to K174; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.3 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 47-55 kDa.
Related Product 1: Nek2-pT170+pS171 blocking peptide
Related Product 2: Nek2-pS171 phosphosite-specific antibody (Cat. No.: AB-PK732)
Scientific Background: Nek2 (NIMA) is a protein-serine/threonine kinase of the Other group and Nek family. It regulates bipolar spindle formation and centrosome separation in mitotic cells, and affects chromatin condensation in meiotic cells. Nek2 can phosphorylate CROCC, CEP250 and NINL, ultimately removing their ability to form a complex in the centrosomes. The proteins mitotic checkpoint proteins CDC20 and MAD2L1 are also phosphorylated and mediated by Nek2. The effect on chromosome condensation is via HMGA2. The ability for Nek2 to activate Nek11 is lost with a K37R mutation. Nek2 kinase phosphotransferase activity can be increased two-fold with a T170E, or S171D, or T175E mutation, while the phosphotransferase activity can be decreased two-fold with a T175A mutation. Complete kinase phosphotransferase activity can be lost with a T179A, T179E, S241A, or S241D mutations. Nek2 is linked to Plexiform Neurofibromas, and chromosome polyploidy. Polyploidy is linked to breast cancer and melanoma, and most often affects smooth muscle, liver, and breast tissues. Nek2 dysfunction has been associated with both primary and secondary breast tumours.
