Product Name:
MST3-pT190
Product Number:
ab-pk728
Target Full Name: Mammalian STE20-like protein-serine kinase 3; Serine-threonine-protein kinase 24
Target Alias: MST3 isoform B; MST3b; RP11-111L24.5; Serine,threonine protein kinase 24; STE20-like kinase MST3; STK24; STK3
Product Type Specific: MST3 protein kinase phosphosite-specific antibody
Antibody Code: PK728
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T190
Protein UniProt: Q9Y6E0
Protein SigNET: MST3
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MST3 (STK24) sequence peptide (Cat. No.: PE-04ABJ95)
Antibody Immunogen Sequence: KRN(pT)FVG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues K187 to G193. T190 phosphorylation stimulates phosphotransferase activity. This phosphosite is located in the kinase activation loop between catalytic subdomains VII and VIII. Phosphorylation regulates cell motility. This is the major in vivo phosphorylation site in MST3 (≥160 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). MST3 is known to be phosphorylated at this site in vitro by MST3 (STK24).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Rat | Mouse | Chicken | Frog | Zebra fish | Fruit fly | Nematode worm.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 50-55 kDa.
Antibody Specificity: Very high
Related Product 1: MST3 (187-193) pT190 antibody blocking peptide (Cat. No.: PE-04ABJ95)
Related Product 2: MstSubtide - MST1 (STK4) protein kinase substrate peptide
Related Product 3: MST3-pT184 phosphosite-specific antibody (Cat. No.: AB-PK727)
Related Product 4: MST1 T183 phosphorylation antibody (Cat. No.: AB-PK871)
Scientific Background: MST3 ((Mammalian Sterile 20-like protein kinase 3; STK24) is a 49 kDa protein-serine/threonine kinase that is a member of the STE group of protein kinases in the STE20 family, and YSK subfamily. This kinase is highly expressed and widely distributed in most tested human tissues, and often localized in the cytoplasm and nucleus. It regulates apoptosis, cell migration, immune response, and metabolism. MST3 promotes apoptosis in response to stress stimuli and caspase activation. It indirectly activates JNK1-JNK2 (MAPK8 and MAPK9), p38 (MAPK11, MAPK12, MAPK13 and MAPK14 ) to mediates oxidative-stress-induced apoptosis. MST3 contains a conserved kinase domain at its N-terminus and a regulatory domain at its C-terminus. Caspase-mediated cleavage of the regulatory domain of MST3 activates its intrinsic kinase activity and leads to its nuclear translocation. Expression of C-terminal truncated MST3 in cells results in DNA fragmentation and induction of apoptosis. MST3 can inhibit cell migration in a fashion dependent on autophosphorylation and can regulate paxillin phosphorylation through protein-tyrosine phosphatase PTP-PEST. It has been implicated a key regulator of axon regeneration in the optic nerve and radial nerve, and in regulation of liver lipid homeostasis. MST3 promotes cancer cell proliferation and migration in certain cancers, such as lung adenocarcinoma. This description may include information annotated by UniProt and/or Google AI.