Product Name:
MST3-pT190
Product Number:
ab-pk728
Target Full Name: Mammalian STE20-like protein-serine kinase 3; Serine-threonine-protein kinase 24
Target Alias: MST3 isoform B; MST3b; RP11-111L24.5; Serine,threonine protein kinase 24; STE20-like kinase MST3; STK24; STK3
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK728
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T190
Protein UniProt: Q9Y6E0
Protein SigNET: Q9Y6E0
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MST3 (STK24) sequence peptide Cat. No.: PE-04ABJ95
Antibody Immunogen Sequence: KRN(pT)FVG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues K187 to G193; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues K187 to G193; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 50-55 kDa.
Antibody Specificity: Very high
Related Product 1: MST3-pT190 blocking peptide
Related Product 2: MST3-pT184 phosphosite-specific antibody (Cat. No.: AB-PK727)
Related Product 3: IRS1 (979-989) KinSub - Insulin receptor substrate 1 (K979-G989, mouse) peptide; Insulin receptor substrate
Related Product 4: MstSubtide - MST1 (STK4) protein kinase substrate peptide
Scientific Background: MST3 (STK24) is a protein-serine/threonine kinase that is a member of the STE group of protein kinases in the STE20 family, and YSK subfamily. This kinase is highly expressed and widely distributed in most tested human tissues. MST3 promotes apoptosis in response to stress stimuli and caspase activation. It indirectly activates JNK1-JNK2 (MAPK8 and MAPK9), p38 (MAPK11, MAPK12, MAPK13 and MAPK14 ) to mediates oxidative-stress-induced apoptosis. MST3 contains a conserved kinase domain at its N-terminus and a regulatory domain at its C-terminus. Caspase-mediated cleavage of the regulatory domain of MST3 activates its intrinsic kinase activity and leads to its nuclear translocation. Expression of C-terminal truncated MST3 in cells results in DNA fragmentation and induction of apoptosis. MST3 can inhibit cell migration in a fashion dependent on autophosphorylation and can regulate paxillin phosphorylation through protein-tyrosine phosphatase PTP-PEST. It has been implicated a key regulator of axon regeneration in the optic nerve and radial nerve.
