Product Name:
Mos-pY263
Product Number:
ab-pk722
Target Full Name: Moloney sarcoma oncogene-encoded protein-serine kinase
Target Alias: ADRB2; C-mos; MGC119963; MSV; MGC119962; CCDS6164.1; ENSG00000172680
Product Type Specific: Mos protein kinase phosphosite-specific antibody
Antibody Code: PK722
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y263
Protein UniProt: P00540
Protein SigNET: Mos
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Mos sequence peptide Cat. No.: PE-04AOC99
Antibody Immunogen Sequence: ADI(pY)SFA(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues A260 to A266; in the protein kinase catalytic domain near subdomain IX. The effect of Y263 phosphorylation is unclear. This is a minor in vivo phosphorylation sites in Mos (≥1 report from high throughput mass spectrometry studies recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse | Chicken | Frog.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 40-45 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in phenylarsine oxide (PAO)+vanadate-treated HeLa (homogenized in SDS-PAGE sample buffer), a strong ~160K cross-reactive protein in EGF-treated A431 cells (homogenized in SDS-PAGE sample buffer); In insulin-treated MCF7 cells, strong background >75 kDa was observed (homogenized in SDS-PAGE sample buffer).
Related Product 1: MOS (260-266) pY263 antibody blocking peptide (Cat. No.: PE-04AOC99)
Related Product 2: Mos (Mos-1) pan-specific antibody (Cat. No.: AB-NK112)
Scientific Background: Mos is a protein-serine/threonine kinase of the Other group and Mos family. During oocyte maturation, it acts as a kinase that directly phosphorylates and activates MEK1 and MEK2 in the MAP kinase signalling module. High levels of Mos are found in germ cells. It is also known as "cytostatic factor" in unfertilized oocytes. It arrests meiosis at metaphase II, to ensure successful egg maturation. Mos is involved in meiotic spindle organization and chromatin organization. It is activated by phosphorylation at Ser-3, and inhibited by phosphorylation at Ser-102. Mos was originally identified as an oncoprotein from avial retroviral studies, where it was discovered as the transforming component (v-mos) of the Moloney murine sarcoma virus (Mo-MSV). It causes aggressive fibrosarcomas in animal models. In human health, alterations like aberrant DNA methylation of the MOS gene are linked to pre-cancerous tissue changes and the long-term development of gastric cancers. In certain cancers, such as lung adenocarcinomas, overexpressed Mos can completely substitute for missing or blocked upstream drivers like EGFR. It does this by activating both the MAPK and PI3K/AKT cell survival pathways. In somatic cells lacking functional p53, elevated Mos protein disrupts the normal coalescence of supernumerary centrosomes. This leads to the formation of multipolar mitotic spindles. The resulting faulty spindle assembly causes unequal chromosome distribution during division, driving gross genomic instability and aneuploidy. Mos is associated with Oocyte/Zygote/Embryo Maturation Arrest 20 and infertility. Female mice deficient in MOS (MOS -/-) show significantly reduced fertility because their oocytes fail to arrest properly at metaphase II. This description may include information annotated by UniProt and/or Google AI.