Product Name:
MOK-pT159+pY161
Product Number:
ab-pk720
Target Full Name: MAPK-MAK-MRK overlapping kinase; MOK protein kinase
Target Alias: RAGE; RAGE1
Product Type Specific: MOK (RAGE) protein kinase phosphosite-specific antibody
Antibody Code: PK720
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T159+Y161
Protein UniProt: Q9UQ07
Protein SigNET: MOK
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MOK (RAGE) sequence peptide Cat. No.: PE-04ABB90
Antibody Immunogen Sequence: QPY(pT)E(pY)IS(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues Q156 to S163; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII. T159 and Y161 phosphorylation stimulate phosphotransferase activity. These are two of the known in vivo phosphorylation sites in MOK, but based on only 1 mass spectrometry report each in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Mouse | Sea urchin.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 47-52 kDa.
Related Product 1: MOK (156-163) pT159+pY161 antibody blocking peptide (Cat. No.: PE-04ABB90)
Related Product 2: MOK-AKCD pan-specific antibody (Cat. No.: AB-NK281-1)
Related Product 3: MOK-pY167 phosphosite-specific antibody (Cat. No.: AB-PK721)
Scientific Background: MOK ((MAPK/MAK/MRK-overlapping kinase; RAGE-1) is an atypical protein-serine/threonine kinase of the CMGC group and RCK family. Phosphorylation appears to increase its enzymatic activity. MOK belongs to the MAP kinase superfamily, and it may phosphorylate exogenous substrates or autophosphorylate. It regulates cell proliferation, differentiation, and cilia growth. The protein inhibits cilium length and intraflagellar transport in renal epithelial cells. It helps manage the balance between proliferation and differentiation in intestinal crypt cells. The MOK gene is expressed in various tissues, including the heart, brain, lung, kidney, and pancreas, with high activity in the intestinal tract. The protein may functions in regulation of cillium length and intraflagellar transport in renal epithelial cells through cAMP and mTORC1 signaling pathways. MOK is directly targeted and regulated by the caudal-related homeobox transcription factor 2 (CDX2). It is localized in the cytoplasm and nucleus, and it plays a key role in intestinal homeostasis and microglial inflammation. MOK controls inflammatory and type-I interferon (IFN) responses in microglia via a Brd4-dependent mechanism, indicating a role in neuroinflammation. MOK is associated with diseases such as Endocrine-Cerebroosteodysplasia and ALS. MOK may be a tumour requiring protein (TRP), since it displays extremely low rates of mutation in human cancers. MOK is a renal tumour antigen that is silent in most normal tissues except for retina and a number of tumour cell types. MOK expression is known to decrease in intestinal adenomas compared to normal tissue. MOK seems to play a role as an important mediator in TDP-43 aggregates exposed to microglia. This description may include information annotated by UniProt and/or Google AI.