Product Name:
MLTK-pT161+pT162
Product Number:
ab-pk719
Target Full Name: Mitogen-activated protein kinase kinase kinase MLT
Target Alias: AZK; HCCS-4; Mixed lineage kinase-related kinase; MLK7; mlklak; MLK-like mitogen-activated protein triple kinase; MLT; MLTK; MRK; MRK-beta; Sterile alpha motif and leucine zipper containing kinase AZK ; MLK7; mlklak; ENSG00000091436
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK719
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T161+T162
Protein UniProt: Q9NYL2
Protein SigNET: Q9NYL2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MLTK (ZAK) sequence peptide Cat. No.: PE-04AQP95
Antibody Immunogen Sequence: HNH(pT)(pT)HMS(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues H158 to S165; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues H158 to S165; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 90-94 kDa.
Related Product 1: MLTK-pT161+pT162 blocking peptide
Scientific Background: MLTK (ZAK) is a protein-serine/threonine kinase of the TKL group and MLK family. It is activated by phosphorylation by PKN1 and autophosphorylation on T161 and S165. It has a pro-apoptotic activity and mediates cell cycle arrest and checkpoint regulation. It regulates the JNK and p38 pathways. Involved in regulation of S and G2 cell cycle checkpoints by direct phosphorylation of Chk2. MLTK causes cell shrinkage and disruption of actin stress fibers and phosphorylates histone H3 at S28. It has been suggested to play a role in cell transformation and oncogenesis.
