Product Name:
MLTK-pT161+pT162
Product Number:
ab-pk719
Target Full Name: Mitogen-activated protein kinase kinase kinase MLT
Target Alias: AZK; HCCS-4; Mixed lineage kinase-related kinase; MLK7; mlklak; MLK-like mitogen-activated protein triple kinase; MLT; MLTK; MRK; MRK-beta; Sterile alpha motif and leucine zipper containing kinase AZK ; MLK7; mlklak; ENSG00000091436
Product Type Specific: MLTK protein kinase phosphosite-specific antibody
Antibody Code: PK719
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T161+T162
Protein UniProt: Q9NYL2
Protein SigNET: MLTK
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MLTK (ZAK) sequence peptide Cat. No.: PE-04AQP95
Antibody Immunogen Sequence: HNH(pT)(pT)HMS(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues H158 to S165; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII. T161 and T162 phosphorylation stimulates phosphotransferase activity. These phosphorylation sites in MLTK have apparently not been reported in high throughput mass spectrometry studies recorded in PhosphoSitePlus. MLTK is known to be phosphorylated in vitro at T161 by ZAK (MLTK), and at T162 by ZAK (MLTK).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus Macaque | Dog | Rat | Mouse | Platypus | Chicken | Zebra fish.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 90-94 kDa.
Scientific Background: MLTK (also known as ZAK, MLTK, or MAP3K20) is a mixed lineage kinase that functions as a Mitogen-Activated Protein Kinase Kinase Kinase (MAP3K). It is a protein-serine/threonine kinase of the TKL group and MLK family. MLTK plays a critical role in cellular stress responses, particularly in mediating signaling pathways that lead to apoptosis (cell death) or cell survival depending on the context.Differential splicing leads to two main isoforms: alpha-ZAK (MLTK-alpha, which contains a long C-terminal portion and a SAM domai.; and beta--ZAK (MLTK-beta), which is a shorter isoform lacking the SAM domain, replaced by a unique tail that allows it to act as a sensor for ribosome collisions and mechanical stress. It is activated by phosphorylation by PKN1 and autophosphorylation on Thr-161 and Ser-165. It has a pro-apoptotic activity and mediates cell cycle arrest and checkpoint regulation. MLTK acts as a sensor for ribosomal collisions, triggering the RSR pathway to induce cell cycle arrest or apoptosis. It regulates the JNK and p38 pathways. Involved in regulation of S and G2 cell cycle checkpoints by direct phosphorylation of Chk2. MLTK causes cell shrinkage and disruption of actin stress fibers and phosphorylates histone H3 at S28. It is found in the cytoplasm and can act as a homodimer. It has been implicated in mediating inflammatory responses and cardiac hypertrophy. It has also been suggested to play a role in cell transformation and oncogenesis. MLTK promotes epithelial-mesenchymal transition (EMT) and is frequently upregulated in colorectal and breast cancers, contributing to poor prognosis. This description may include information annotated by UniProt and/or Google AI.