Product Name:
MLK3-pS281
Product Number:
ab-pk718
Target Full Name: Mixed-lineage protein-serine kinase 3; Mitogen-activated protein kinase kinase kinase 11
Target Alias: Kinase MLK3; M3K11; MAP3K11; MAPKKK11; MEKK11; Mitogen activated protein kinase kinase kinase 11; Mixed lineage kinase 3; MLK-3; SPRK; MGC17114; CCDS8107.1; ENSG00000173327
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK718
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S281
Protein UniProt: Q16584
Protein SigNET: Q16584
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MLK3 (MAP3K11) sequence peptide Cat. No.: PE-04AOB85
Antibody Immunogen Sequence: TQM(pS)AAG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues T278 to G284; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues T278 to G284; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 93-100 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: No significant cross-reactivities detected in HeLa cells treated with phenylarsine oxide (PAO)+vanadate (homogenized in SDS-PAGE sample buffer), except for a strong 50kDa cross-reactive protein ; medium immunoreactive 50 kDa protein in EGF-treated A431cells (SDS-PAGE buffer-extracted); Clean, but strong background >75K in insulin-treated MCF7 cells (SDS-PAGE buffer extracted).
Related Product 1: MLK3-pS281 blocking peptide
Related Product 2: MLK3-pT277 + pS281 phosphosite-specific antibody (Cat. No.: AB-PK870)
Scientific Background: MLK3 (MAP3K11) is a protein-serine/threonine kinase of the TKL group and MLK family. It induces the indirect activation of B-Raf, MKK4 (MAP2K4), MKK7 (MAP2K7), JNK1 (MAPK8), MAPK14 and ERK1 (MAPK3). Homodimerization via the leucine zipper domains is required for autophosphorylation at T277 and S281 and increases phosphotransferase activity.