Product Name:
MLK3-pS281
Product Number:
ab-pk718
Target Full Name: Mixed-lineage protein-serine kinase 3; Mitogen-activated protein kinase kinase kinase 11
Target Alias: Kinase MLK3; M3K11; MAP3K11; MAPKKK11; MEKK11; Mitogen activated protein kinase kinase kinase 11; Mixed lineage kinase 3; MLK-3; SPRK; MGC17114; CCDS8107.1; ENSG00000173327
Product Type Specific: MLK3 protein kinase phosphosite-specific antibody
Antibody Code: PK718
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S281
Protein UniProt: Q16584
Protein SigNET: MLK3
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MLK3 (MAP3K11) sequence peptide Cat. No.: PE-04AOB85
Antibody Immunogen Sequence: TQM(pS)AAG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues T278 to G284; in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. S281 phosphorylation stimulates phosphotransferase activity. This is one of the major in vivo phosphorylation sites in MLK3. MLK3 is known to be phosphorylated at this site in vitro by HPK1 (MAP4K1), and MLK3 (MAP3K11).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Rat | Mouse | Frog | Zebra fish | Honey bee.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 93-100 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: No significant cross-reactivities detected in HeLa cells treated with phenylarsine oxide (PAO)+vanadate (homogenized in SDS-PAGE sample buffer), except for a strong 50kDa cross-reactive protein ; medium immunoreactive 50 kDa protein in EGF-treated A431cells (SDS-PAGE buffer-extracted); Clean, but strong background >75K in insulin-treated MCF7 cells (SDS-PAGE buffer extracted).
Related Product 1: MLK3 (278-284) pS281 antibody blocking peptide (Cat. No.: PE-04AOB85)
Related Product 2: MLK1 expression antibody (Cat. No.: AB-NK278-1)
Related Product 3: MLK4 expression antibody (Cat. No.: AB-NK280-1)
Scientific Background: MLK3 (MAP3K11) is a protein-serine/threonine kinase of the TKL group and MLK family. It acts as a positive regulator of the JNK, p38, and ERK signaling pathways, playing a key role in cell proliferation, migration, apoptosis, and immune responses, including enhanced T-cell toxicity against cancer cells. It is activated by Rho family GTPases (Cdc42/Rac1) and Raf-1. It induces the indirect activation of B-Raf, MKK4 (MAP2K4), MKK7 (MAP2K7), JNK1 (MAPK8), MAPK14 and ERK1 (MAPK3). It preferentially activates the JNK (c-Jun NH2-terminal kinase) pathway by phosphorylating MAP2K4/MKK4 and MAP2K7/MKK7. It activates p38 MAP kinase and, to a lesser extent, the ERK pathway. It also regulates NF-κB by activating IKK complexes and influences microtubule organization. It is involved in regulating actin cytoskeleton remodeling and cell migration, often by interacting with proteins like Galphaq and p63RhoGEF/GEFT. Homodimerization via the leucine zipper domains is required for autophosphorylation at Thr-277 and Ser-281 and increases phosphotransferase activity. It features an SH3 domain, a leucine zipper motif, and a Cdc42/Rac-interactive binding (CRIB) motif. It is largely resides in the cytoplasm, where it can function as a scaffold for signaling complexes, but it can also localize to the centrosomal region. MLK3 is often overexpressed in tumours (e.g., breast, ovarian, glioma), promoting cancer cell survival, invasion, and metastasis. Inhibition of MLK3 has been shown to induce T-cell activation and inhibit tumour growth. This description may include information annotated by UniProt and/or Google AI.