Product Name:
MKK3-pS218
Product Number:
ab-pk713
Target Full Name: MAPK/ERK protein-serine kinase 3 beta isoform (MKK3 beta); Dual specificity mitogen-activated protein kinase kinase 3
Target Alias: MAP kinase kinase 3; MAPK,ERK kinase 3; MAPK/ERK kinase 3; MAPKK 3; MAP2K3; MP2K3; PRKMK3; P46734; ENSG00000034152
Product Type Specific: MKK3 protein kinase phosphosite-specific antibody
Antibody Code: PK713
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S218
Protein UniProt: P46734
Protein SigNET: MKK3
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MKK3 (MAP2K3, MEK3) sequence peptide Cat. No.: PE-04ABS99
Antibody Immunogen Sequence: YLVD(pS)VAK(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues Y214 to K221; in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. S218 phosphorylation stimulates phosphotransferase activity. This is one of the major in vivo phosphorylation sites in MKK3 (≥74 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). MKK3 is known to be phosphorylated at this site in vitro by ASK1 (MAP3K5), COT (MAP3K8), MEKK3 (MAP3K3), MKK3 (MAP2K3, MEK3), and MLK3 (MAP3K11).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Dog | Mouse | Platypus | Frog.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 37-40 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: Strongly immunoreactivity on protein dot blots with human recombinant MKK4, but not MEK1 and MKK6.
Related Product 1: MEK3 (214-221) pS218 antibody blocking peptide (Cat. No.: PE-04ABS99)
Related Product 2: MKK3-1 pan-specific antibody (Cat. No.: AB-NK101-4)
Related Product 3: MKK3 expression antibody (Cat. No.: AB-NK101-4)
Related Product 4: MKK3 expression antibody (Cat. No.: AB-NK101-6)
Related Product 5: MKK3 S218 phosphorylation antibody (Cat. No.: AB-PK713)
Related Product 6: MKK6 expression antibody (Cat. No.: AB-NK105-3)
Related Product 7: MKK6 expression antibody (Cat. No.: AB-NK105-4)
Related Product 8: MKK6 expression antibody (Cat. No.: AB-NK105-5)
Scientific Background: MKK3 (MAP2K3, MEK3) is a Dual specificity protein kinase of the STE group and STE7 family. It is activated by environmental stress, inflammatory cytokines, and growth factors, and functions as a crucial regulator of cell differentiation, apoptosis, and tumour progression, particularly in breast cancer and glioma. Phosphorylation of Ser-218 and Thr-222 increases its phosphotransferase activity. S218A and T222A substitutions are involved in inactivation. S218E and T222E mutations are associated in constitutive activation. It catalyzes the concomitant phosphorylation of threonine and tyrosine residues (e.g., Thr-180 and Tyr-182 in p38-alpha) in the activation T loops of the p38 MAP kinase isoforms. It is widely expressed in many tissues, and abundant in skeletal muscle. MKK3 is crucial for p38-mediated cytokine release in immune responses. It is targeted by pathogens, such as Yersinia pseudotuberculosis, to evade the host immune system.MKK3 inhibition, especially in p53-mutant cancers, can reduce tumour proliferation and induce apoptosis. Defects in the MKK3 gene may be involved colon cancer. This description may include information annotated by UniProt and/or Google AI.