Product Name:
MKK3-pS218
Product Number:
ab-pk713
Target Full Name: MAPK/ERK protein-serine kinase 3 beta isoform (MKK3 beta); Dual specificity mitogen-activated protein kinase kinase 3
Target Alias: MAP kinase kinase 3; MAPK,ERK kinase 3; MAPK/ERK kinase 3; MAPKK 3; MAP2K3; MP2K3; PRKMK3; P46734; ENSG00000034152
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK713
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S218
Protein UniProt: P46734
Protein SigNET: P46734
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MKK3 (MAP2K3, MEK3) sequence peptide Cat. No.: PE-04ABS99
Antibody Immunogen Sequence: YLVD(pS)VAK(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues Y214 to K221; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues Y214 to K221; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 37-40 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: Strongly immunoreactivity on protein dot blots with human recombinant MKK4, but not MEK1 and MKK6.
Related Product 1: MKK3-pS218 blocking peptide
Related Product 2: MKK3-1 pan-specific antibody (Cat. No.: AB-NK101-4)
Related Product 3: MKK3-2 pan-specific antibody (Cat. No.: AB-NK101-5)
Related Product 4: MKK3-3 pan-specific antibody (Cat. No.: AB-NK101-6)
Related Product 5: MKK3-pY230 phosphosite-specific antibody (Cat. No.: AB-PK714)
Scientific Background: MKK3 (MAP2K3, MEK3) is a Dual specificity protein kinase of the STE group and STE7 family. MKK3 is activated by cytokines and environmental stress in vivo. Phosphorylation of S218 and T222 increases its phosphotransferase activity. S218A and T222A substitutions are involved in inactivation. S218E and T222E mutations are associated in constitutive activation. It catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the activation T loops of the p38 MAP kinase isoforms. Defects in the MKK3 gene may be involved colon cancer.
