Product Name:
Met-pY1234+pY1235
Product Number:
ab-pk711
Target Full Name: Hepatocyte growth factor (HGF) receptor-tyrosine kinase
Target Alias: C-met; Hepatocyte growth factor receptor; HGF receptor; HGFR; HGF-SF receptor; Kinase Met; Met proto- oncogene tyrosine kinase; Met proto-oncogene; RCCP2; ENSG00000105976
Product Type Specific: Met protein kinase phosphosite-specific antibody
Antibody Code: PK711
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y1234+Y1235
Protein UniProt: P08581
Protein SigNET: Met
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Met sequence peptide Cat. No.: PE-04AFO99
Antibody Immunogen Sequence: KE(pY)(pY)SVH(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues K1232 to H1238; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII. Y1234 and Y1235 phosphorylation stimulate phosphotransferase activity. Y1234 and Y1235 phosphorylation induces binding to PTPN1 and PTPN2, and Y1234 phosphorylation also induces interaction with Grb2. These are two of the major in vivo phosphorylation sites in Met (≥771 and ≥453 reports, respectively, from high throughput mass spectrometry studies recorded in PhosphoSitePlus). Met is known to be phosphorylated in vitro at Y1234 by Met, and Ron (MST1R), and at Y1235 by Met, and Ron (MST1R).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Rat | Mouse | Platypus | Tiger blowfish.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 140-155 kDa.
Related Product 1: Met (1232-1238) pY1234+pY1235 antibody blocking peptide (Cat. No.: PE-04AFO99)
Related Product 2: MetSubtide kinase substrate peptide (Cat. No.: PE-01BID99)
Related Product 3: Met expression antibody (Cat. No.: AB-NK110-4)
Related Product 4: Met S1236 phosphorylation antibody (Cat. No.: AB-PK705)
Related Product 5: Met T1241 phosphorylation antibody (Cat. No.: AB-PK706)
Related Product 6: Met T1355+Y1356 phosphorylation antibody (Cat. No.: AB-PK707)
Related Product 7: Met Y1003 phosphorylation antibody (Cat. No.: AB-PK708)
Related Product 8: Met Y1230 phosphorylation antibody (Cat. No.: AB-PK709)
Related Product 9: Met Y1234 phosphorylation antibody (Cat. No.: AB-PK710)
Related Product 10: Met Y1234+Y1235 +S1236 phosphorylation antibody (Cat. No.: AB-PK712)
Scientific Background: Met (HGFR) is a protein-tyrosine kinase of the TK group and Met family. Met is produced as a single-chain precursor, then cleaved to form a disulfide-linked heterodimer: a 50 kDa extracellular alpha subunit, and 145 kDa beta subunit with a Sema domain, a PSI domain, and an intracellular region containing a juxtamembrane domain, a tyrosine kinase domain, and a docking site. It is activated by binding hepatocyte growth factor (HGF) to the Sema domain, and this induces dimerization and autophosphorylation. Phosphorylation of Tyr-1003 increases phosphotransferase activity and induces interaction with Cbl. Phosphorylation of Tyr-1230 and Tyr-1235 increases phosphotransferase activity. Phosphorylation of Tyr-1234 increases phosphotransferase activity and induces interaction with Grb2. Phosphorylation of S985 inhibits phosphotransferase activity. Phosphorylation of Tyr-1349 inhibits phosphotransferase activity, but induces interaction with FAK, Gab1, Grb2, Met, PIK3R1, PLCG1 and Src. Met mediates scattering, proliferation, survival, and morphogenesis of cells. Some immediate regulators that bind to Met after activation with HGF include PLCG1, PIK3R1, GRB2, SRC, STAT3 or the adapter GAB1. Met is a known oncoprotein. Cancer-related mutations in human tumours point to a gain of function of the protein kinase. Overexpression, amplification, or mutation drives various cancers, including non-small cell lung cancer (NSCLC), gastric cancer, and kidney cancer. Hepatocellular carcinoma (HCC) also occurs with higher risk in those that have chronic HCV (hepatitis C virus) or HBV (hepatitis B virus) infection, or aflatoxin exposure in diet, or alcohol-induced cirrhosis, or another form of cirrhosis. This description may include information annotated by UniProt and/or Google AI.