Product Name:
Met-pY1230
Product Number:
ab-pk709
Target Full Name: Hepatocyte growth factor (HGF) receptor-tyrosine kinase
Target Alias: C-met; Hepatocyte growth factor receptor; HGF receptor; HGFR; HGF-SF receptor; Kinase Met; Met proto- oncogene tyrosine kinase; Met proto-oncogene; RCCP2; ENSG00000105976
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK709
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y1230 (not Y1234
Protein UniProt: P08581
Protein SigNET: P08581
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Met sequence peptide Cat. No.: PE-04AAW70
Antibody Immunogen Sequence: M(pY)DKEYY(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues M1229 to Y1235; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues M1229 to Y1235; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots. Very strong immunoreactivity with recombinant human Met on protein dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 140-155 kDa.
Antibody Specificity: Medium
Antibody Cross Reactivity: Weak immunoreactivity on protein dot blots with recombinant human Ron. In HEK-293 cells, phenylarsine oxide (PAO) decreases 50, 30 and 16 kDa cross-reactive protein detection. in Jurkat cells, phenylarsine oxide (PAO) increases detection of 110, 90, 60 and 15 kDa proteins.
Related Product 1: Met-pY1230 blocking peptide
Related Product 2: Met-1 pan-specific antibody (Cat. No.: AB-NK110-2)
Related Product 3: Met-3 pan-specific antibody (Cat. No.: AB-NK110-4)
Related Product 4: Met-pS1236 phosphosite-specific antibody (Cat. No.: AB-PK705)
Related Product 5: Met-pT1241 phosphosite-specific antibody (Cat. No.: AB-PK706)
Related Product 6: Met-pT1355+pY1356 phosphosite-specific antibody (Cat. No.: AB-PK707)
Related Product 7: Met-pY1003 phosphosite-specific antibody (Cat. No.: AB-PK708)
Related Product 8: Met-pY1234 phosphosite-specific antibody (Cat. No.: AB-PK710)
Related Product 9: Met-pY1234+pY1235 phosphosite-specific antibody (Cat. No.: AB-PK711)
Related Product 10: MetSubtide - Met protein kinase substrate peptide
Scientific Background: Met (HGFR) is a protein-tyrosine kinase of the TK group and Met family. It is activated by binding hepatocyte growth factor (HGF), which induces dimerization and autophosphorylation. Phosphorylation of Y1003 increases phosphotransferase activity and induces interaction with Cbl. Phosphorylation of Y1230 and Y1235 increases phosphotransferase activity. Phosphorylation of Y1234 increases phosphotransferase activity and induces interaction with Grb2. Phosphorylation of S985 inhibits phosphotransferase activity. Phosphorylation of Y1349 inhibits phosphotransferase activity, but induces interaction with FAK, Gab1, Grb2, Met, PIK3R1, PLCG1 and Src. Met mediates scattering, proliferation, survival, and morphogenesis of cells. Some immediate regulators that bind to Met after activation with HGF include PLCG1, PIK3R1, GRB2, SRC, STAT3 or the adapter GAB1. Met is a known oncoprotein (OP). Cancer-related mutations in human tumours point to a gain of function of the protein kinase. The active form of the protein kinase normally acts to promote tumour cell proliferation. Hepatocellular carcinoma (HCC) occurs with higher risk in those that have chronic HCV (hepatitis C virus) or HBV (hepatitis B virus) infection, or aflatoxin exposure in diet, or alcohol-induced cirrhosis, or another form of cirrhosis.
