Product Name:
MERTK-pY749+pY753
Product Number:
ab-pk703
Target Full Name: Tyrosine-protein kinase Mer; Proto-oncogene tyrosine-protein kinase MER
Target Alias: C-mer; C-mer proto-oncogene tyrosine kinase; Kinase Mer; MERK; MERTK; MGC133349Proto-oncogene tyrosine-protein kinase MER precursor; Receptor tyrosine kinase MerTK; RP38; MGC133349; CCDS2094.1; ENSG00000153208
Product Type Specific: MERTK protein kinase phosphosite-specific antibody
Antibody Code: PK703
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y749+Y753
Protein UniProt: Q12866
Protein SigNET: MERTK
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MERTK (MER) sequence peptide Cat. No.: PE-04AAV85
Antibody Immunogen Sequence: KI(pY)SGD(pY)Y(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues K747 to Y754; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII. Y749 and Y753 phosphorylation stimulate phosphotransferase activity. This is one of the major in vivo phosphorylation sites in MERTK (≥449 and ≥403 reports, respectively, from high throughput mass spectrometry studies recorded in PhosphoSitePlus). MERTK is known to be phosphorylated in vitro at Y749 by MER (MERTK), and at Y753 by MER (MERTK).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 105-115 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in HeLa cells.
Related Product 1: MERTK (747-754) pY749+pY753 antibody blocking peptide (Cat. No.: PE-04AAV85)
Related Product 2: MERTK-pY749 phosphosite-specific antibody (Cat. No.: AB-PK702)
Related Product 3: MERTK-pY753 phosphosite-specific antibody (Cat. No.: AB-PK704)
Related Product 4: MerSubtide - Mer (MERTK) protein kinase substrate peptide
Scientific Background: MERTK (MER) is a protein-tyrosine kinase of the TK group and Axl family. It is a receptor kinase that activates downstream signaling (e.g., PI3K/Akt, MAPK/ERK) upon binding ligands such as Gas6, Tubby, or Galectin-3 . It functions in the regulation of cell survival, angiogenesis, migration and invasion, differentiation, and promotes non-inflammatory phagocytosis of apoptotic cells and suppresses inflammation. It is critical for phagocytosis of rod outer segments by the retinal pigment epithelium (RPE). It features two Ig-like domains and two FNIII domains, and anchored in the plasma membrane with a transmembrane domain. Phosphorylation of Tyr-749, Tyr-753 and Tyr-754 increases phosphotransferase activity. It is highly expressed in macrophages, dendritic cells, NK cells, and epithelial cells. Mutations cause retinitis pigmentosa, and overexpression is linked to various leukemias and solid tumours. This description may include information annotated by UniProt and/or Google AI.