Product Name:
MEKK2-pS239
Product Number:
ab-pk700
Target Full Name: MAPK/ERK kinase kinase 2; Mitogen-activated protein kinase kinase kinase 2
Target Alias: Kinase MEKK2; M3K2; MAP/ERK kinase kinase 2; MAP3K2; MAPK/ERK kinase kinase 2; MAPKKK2; MEK kinase 2; MEKK 2; MEKK2B; ENSG00000169967
Product Type Specific: MEKK2 protein kinase phosphosite-specific antibody
Antibody Code: PK700
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S239
Protein UniProt: Q9Y2U5
Protein SigNET: MEKK2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MEKK2 (MAP3K2) sequence peptide Cat. No.: PE-04ANX90
Antibody Immunogen Sequence: RAQ(pS)YPD(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R236 to D242; in the region between the PB1 domain and the protein kinase catalytic domain. The effect of S239 phosphorylation is unclear. This is a major site of phosphorylation for MEKK2 (≥281 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Mouse | Chicken.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 66-74 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: Almost no significant cross-reactivities detected in HeLa cells.
Related Product 1: MEKK2 (236-242) pS239 antibody blocking peptide (Cat. No.: PE-04ANX90)
Related Product 2: MEKK2-1 pan-specific antibody (Cat. No.: AB-NK108-3)
Related Product 3: MEKK2-2 pan-specific antibody (Cat. No.: AB-NK108-4)
Related Product 4: MEKK2-3 pan-specific antibody (Cat. No.: AB-NK108-5)
Related Product 5: MEKK6-2 pan-specific antibody (Cat. No.: AB-NK225-2)
Related Product 6: MEKK6-3 pan-specific antibody (Cat. No.: AB-NK225-3)
Scientific Background: MEKK2 (MAP3K2) is a 70 kDa protein-serine/threonine kinase of the STE group and STE11 family. It functions as a critical MAP3K in signaling pathways regulating immune response, inflammation, and cell proliferation. It acts as an upstream kinase, primarily activating the JNK1/2, p38, ERK5, and NF-κB pathways, but It also binds to Protein Kinase C-related Kinase 2 (PRK2). It is required for cytokine production in cultured mast cells. The protein is one of the kinases that regulate different steps of the caveolar cycle. It features a C-terminal catalytic serine/threonine kinase domain and a distinct N-terminal regulatory domain. MEKK2 activity is regulated through 14-3-3 protein binding, which modulates dimerization. It is also activated by phosphorylation on Ser-514, Ser-520, and Thr-522. It is also known to form a complex with Mip1, which acts as an inhibitor. Methylation of the protein increases MAP kinase signalling and promotes Ras-driven carcinomas. Overexpression of MEKK2 has been associated with colorectal cancer progression, where it typically resides in the cytoplasm. This description may include information annotated by UniProt and/or Google AI.