Product Name:
MEK5-pS311
Product Number:
ab-pk699
Target Full Name: MAPK/ERK protein-serine kinase 5 (MKK5); Dual specificity mitogen-activated protein kinase kinase 5
Target Alias: CXA1; HsT17454; MAP kinase kinase 5; MAP2K5; MAPK,ERK kinase 5; MAPK/ERK kinase 5; MAPKK5; MKK5; PRKMK5; HsT17454; ENSG00000137764
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK699
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S311
Protein UniProt: Q13163
Protein SigNET: Q13163
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MEK5 (MAP2K5, MKK5) sequence peptide Cat. No.: PE-04ANV99
Antibody Immunogen Sequence: LVN(pS)IAK(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues L308 to K314; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues L308 to K314; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 47-52 kDa.
Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant humanMEK1, MEK2, MKK4 or MKK6.
Related Product 1: MEK5-pS311 blocking peptide
Related Product 2: MEK5-1 pan-specific antibody (Cat. No.: AB-NK104-3)
Related Product 3: MEK5-2 pan-specific antibody (Cat. No.: AB-NK104-4)
Related Product 4: MEK5-3 pan-specific antibody (Cat. No.: AB-NK104-5)
Related Product 5: MEK5-PNT (MEK5-4) pan-specific antibody (Cat. No.: AB-NK104-2)
Scientific Background: MEK5 (MAP2K5) is a Dual specificity protein kinase of the STE group and STE7 family. It is activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases. Activation of this pathway appear to play a critical role in protecting cells from stress-induced apopotosis, neuronal survival and cardiac development and angiogenesis by acting as a scaffold protein for the construction of a complex consisting of MAP3K2 or MAP3K3 and MAP3K5 and MAPK7.
