Product Name:
MEK1-pS222
Product Number:
ab-pk698
Target Full Name: MAPK/ERK protein-serine kinase 1; Dual specificity mitogen-activated protein kinase kinase 1
Target Alias: ERK activator kinase 1; Kinase MEK1; MAP kinase kinase 1; MAP2K1; MAPK,ERK kinase 1; MAPK/ERK kinase 1; MAPKK 1; MAPKK1; MKK1; PRKMK1; CCDS10216.1; Q02750; ENSG00000169032
Product Type Specific: MEK1 protein kinase phosphosite-specific antibody
Antibody Code: PK698
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S222
Protein UniProt: Q02750
Protein SigNET: MEK1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MEK1 (MKK1, MAP2K1) sequence peptide Cat. No.: PE-04AEC95
Antibody Immunogen Sequence: MAN(pS)YIC(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues M219 to S222. S222 phosphorylation stimulates phosphotransferase activity. This phosphosite is located in the kinase activation loop between catalytic subdomains VII and VIII. This is the major in vivo phosphorylation sites in MEK1 (≥213 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). MEK1 is known to be phosphorylated at this site in vitro by A-Raf (RafA), B-Raf (RafB), COT (MAP3K8), KSR2, MEK1 (MAP2K1), MEKK1 (MAP3K1), Mos, PDPK1 (PDK1), Plk1, and Raf1.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse | Platypus | Chicken |Frog | Fruit fly |Nematode worm | Sea urchin.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 44-47 kDa.
Related Product 1: MEK1 (219-222) pS222 antibody blocking peptide (Cat. No.: PE-04AEC95)
Related Product 2: MEKSubtide kinase substrate peptide (Cat. No.: PE-01BIB75)
Related Product 3: MEK1 expression antibody (Cat. No.: AB-NK099-2)
Related Product 4: MEK1 expression antibody (Cat. No.: AB-NK099-3)
Related Product 5: MEK1 expression antibody (Cat. No.: AB-NK099-4)
Related Product 6: MEK1 expression antibody (Cat. No.: AB-NK099-7)
Related Product 7: MEK1 expression antibody (Cat. No.: AB-NK099-8)
Scientific Background: MEK1 (MAP2K1) is a 43-45 kDa Dual specificity protein kinase of the STE group and STE7 family. It functions in the MAP kinase signalling pathway, and plays a role in the regulation of cell growth, adhesion, survival, and differentiation. It is a dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates Ras and this initiates Raf1 activation. Raf1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Phosphorylation of Ser-218 and Ser-222 in MEK1 activates phosphotransferase activity, phosphorylation at Ser-212 and Thr-286 is inhibitory, and phosphorylation at Thr-292 increases molecular association with ERK2 and Raf1, but reduces interaction with ERK1. It is inhibited by feedback phosphorylation at Thr-292 by active ERK1/2, and its phosphorylation on Ser-298 by PAK is regulated by cell adhesion. Phosphorylation of Thr-386 also induces interaction with ERK2. Overactive MEK1, often driven by mutations in the MAP2K1 gene or upstream elements (RAS/RAF), is linked to cancer cell growth. This description may include information annotated by UniProt and/or Google AI.