Product Name:
MEK1-pS222
Product Number:
ab-pk698
Target Full Name: MAPK/ERK protein-serine kinase 1 (MKK1); Dual specificity mitogen-activated protein kinase kinase 1
Target Alias: ERK activator kinase 1; Kinase MEK1; MAP kinase kinase 1; MAP2K1; MAPK,ERK kinase 1; MAPK/ERK kinase 1; MAPKK 1; MAPKK1; MKK1; PRKMK1; CCDS10216.1; Q02750; ENSG00000169032
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK698
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S222
Protein UniProt: Q02750
Protein SigNET: Q02750
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MEK1 (MKK1, MAP2K1) sequence peptide Cat. No.: PE-04AEC95
Antibody Immunogen Sequence: MAN(pS)YIC(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues M219 to S222; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues M219 to S222; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 44-47 kDa.
Related Product 1: MEK1-pS222 blocking peptide
Related Product 2: MEK1-1 pan-specific antibody (Cat. No.: AB-NK099-8)
Related Product 3: MEK1-3 (MEK1-7) pan-specific antibody (Cat. No.: AB-NK099-7)
Related Product 4: MEK1-CT pan-specific antibody (Cat. No.: AB-NK099-2)
Related Product 5: MEK1-pS222 phosphosite-specific antibody (Cat. No.: AB-PK698)
Related Product 6: MEKSubtide - MEK1 (MAP2K1) protein kinase substrate peptide
Scientific Background: MEK1 (MAP2K1) is a Dual specificity protein kinase of the STE group and STE7 family. It functions in the MAP kinase signalling pathway, and plays a role in the regulation of cell growth, adhesion, survival, and differentiation. Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates Ras and this initiates Raf1 activation. Raf1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Phosphorylation of S212 and T286 in MEK1 inhibits phosphotransferase activity, and phosphorylation at T292 increases molecular association with ERK2 and Raf1, but reduces interaction with ERK1. Phosphorylation of T386 induces interaction with ERK2.
