Product Name:
MARK1-pT215
Product Number:
ab-pk694
Target Full Name: MAP/microtubule affinity-regulating protein-serine kinase 1
Target Alias: KIAA1477; MAP/microtubule affinity-regulating kinase 1; MARK
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK694
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T215
Protein UniProt: Q9P0L2
Protein SigNET: Q9P0L2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MARK1 sequence peptide Cat. No.: PE-04AAT95
Antibody Immunogen Sequence: NKLD(pT)F(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues K212 to G218; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII. This is the major in vivo phosphorylation site in MARK1.
Production Method: Corresponds to amino acid residues K212 to G218; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII. This is the major in vivo phosphorylation site in MARK1.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 85-90 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: Strong 46K cross-reactive protein (Nim1, LKB and ATP6V1C2 are candidates).
Related Product 1: MARK1-pT215 blocking peptide
Related Product 2: MARK1-ANT pan-specific antibody (Cat. No.: AB-NK098-2)
Related Product 3: MARK1-pT215 phosphosite-specific antibody (Cat. No.: AB-PK695)
Related Product 4: Cdc25C (205-225), KinSub - Cdc25C phosphatase (V205-R225, human) peptide; Chktide peptide
Related Product 5: MARK2-ANT pan-specific antibody (Cat. No.: AB-NK275-1)
Related Product 6: MARK2-BCT pan-specific antibody (Cat. No.: AB-NK275-2)
Related Product 7: MARK3-ANT pan-specific antibody (Cat. No.: AB-NK276-1)
Related Product 8: MARK3-BCT pan-specific antibody (Cat. No.: AB-NK276-2)
Related Product 9: MARK3-pT507 phosphosite-specific antibody (Cat. No.: AB-PK697)
Related Product 10: MARK4-ANT pan-specific antibody (Cat. No.: AB-NK277-1)
Scientific Background: MARK1 is a protein-serine/threonine kinase that is is a member of the CAMK group of protein kinases in the CAMKL family, and MARK subfamily. It mediates control of cell polarity and microtubule function. This kinase is highly expressed and widely distributed in most tested human tissues except apparently in the brain and spinal cord. It is activated by phosphorylation on T215 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. MARK1 phosphorylates microtubule-associated proteins and trigger microtubule disruption. Gene mutation studies performed in mice revealed that after targeted disruption of the MARK1 gene, the mice lacked the ability to drink, and displayed hind leg motor dysfunction. Genetic variations in MARK1 have been correlated with susceptibility to autism. MARK1 is over-expressed in the prefrontal cortex of autism patients and affects the function of cortical dendrites. MARK1 has also been linked with the development of gastric adenocarcinomas and ovarian serous carcinomas.
