Product Name:
MAPKAPK5-pT186
Product Number:
ab-pk693
Target Full Name: MAP kinase-activated protein kinase 5
Target Alias: MAP kinase-activated protein kinase 5; MAPKAP kinase 5; PRAK
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK693
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T186
Protein UniProt: Q8IW41
Protein SigNET: Q8IW41
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MAPKAPK5 (PRAK) sequence peptide Cat. No.: PE-04AAS95
Antibody Immunogen Sequence: PQF(pT)PYYV(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues P183 to V190; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues P183 to V190; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 53-58 kDa.
Antibody Specificity: Very high
Related Product 1: MAPKAPK5-pT186 blocking peptide
Related Product 2: MAPKAPK2-pT222 phosphosite-specific antibody (Cat. No.: AB-PK690)
Related Product 3: MAPKAPK2-pY225+pT226 phosphosite-specific antibody (Cat. No.: AB-PK691)
Related Product 4: MAPKAPK3-pY76 phosphosite-specific antibody (Cat. No.: AB-PK692)
Related Product 5: HSP27 (81-86) KinSub - Based on Heat shock protein 27 (L81-S86, mouse) peptide; HSP27tide protein kinase substrate peptide
Related Product 6: MAPKAPK3Subtide - MAPKAPK2 protein kinase substrate peptide
Scientific Background: MAPKAPK5 is a protein-serine/threonine kinase of the CAMK group and MAPKAPK family. p38 alpha and beta-dependent phosphorylation increases its activity. It is activated by stress-related extracellular stimuli; such as H2O2, arsenite, anisomycin TNF alpha and also PMA and the calcium ionophore A23187; but to a lesser extent. In vitro, activated by SQSTM1. T182A substitution leads to no p38-beta/MAPK11-induced activation. In addition, T182D, S212D and L337G mutations are associated with higher protein kinase activity. It appears to be a tumour suppressor protein (TSP) that mediates Ras-induced senescence and phosphorylates p53/TP53. It also phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, and RHEB.
