Product Name:
MAK-pT157
Product Number:
ab-pk689
Target Full Name: Male germ cell-associated protein-serine kinase
Target Alias: Male germ cell-associated kinase
Product Type Specific: MAK protein kinase phosphosite-specific antibody
Antibody Code: PK689
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T157
Protein UniProt: P20794
Protein SigNET: MAK
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MAK sequence peptide Cat. No.: PE-04ADW90
Antibody Immunogen Sequence: PPY(pT)DYV(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues P154 to V160. T157 phosphorylation stimulates phosphotransferase activity. This phosphosite is located in the kinase activation loop between catalytic subdomains VII and VIII. This is one of the major in vivo phosphorylation sites in MAK (≥617 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). MAK is known to be phosphorylated at this site in vitro by MAK.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse | Platypus | Chicken | Zebra fish.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 80-85 kDa.
Antibody Specificity: High-very high
Related Product 1: MAK (154-160) pT157 antibody blocking peptide (Cat. No.: PE-04ADW90)
Scientific Background: MAK (Male Germ Cell-Associated Kinase) is a protein-serine/threonine kinase of the CMGC group and RCK family, and is involved in regulating cell cycle progression, microtubule-organizing centers, and spermatogenesis. MAK is essential for male fertility, as it is highly expressed in germ cells at and after meiosis. It has been associated with maintaining ciliary length (ciliogenesis) and modulating long-term photoreceptor cell survival. MAK has been shown to phosphorylate FZR1 during different stages of cell cycle. MAK may have a role in prostate cancer chromosome stability and in spermatogenesis. It is located in the connecting cilia and outer segment axonemes of photoreceptors, and in the nuclei and centrosomes during the cell cycle. It has been associated with retinal degeneration, with mutations causing retinitis pigmentosa 62. This description may include information annotated by UniProt and/or Google AI.