Product Name:
Lyn-pY508
Product Number:
ab-pk688
Target Full Name: Yes-related protein-tyrosine kinase; Tyrosine-protein kinase Lyn
Target Alias: JTK8; Kinase Lyn; KPM; Lyn; V-yes-1 Yamaguchi sarcoma viral related oncogene; FLJ26625; CCDS6162.1; ENSG00000147507
Product Type Specific: Lyn protein kinase phosphosite-specific antibody
Antibody Code: PK688
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y508
Protein UniProt: P07948
Protein SigNET: Lyn
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Lyn sequence peptide Cat. No.: PE-04ANN90
Antibody Immunogen Sequence: EGQ(pY)QQQ(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues E505 to Q511; Near the C-terminus of the kinase. Y508 phosphorylation inhibits phosphotransferase activity. This is one of the major in vivo phosphorylation sites in Lyn (≥1583 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). Lyn is known to be phosphorylated at this site in vitro by CSK, and CTK (MATK).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse | Platypus | Chicken | Frog | Zebra fish | Fruit fly.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 55-60 kDa.
Related Product 1: Lyn (505-511) pY508 antibody blocking peptide (Cat. No.: PE-04ANN90)
Related Product 2: LynSubtide kinase substrate peptide (Cat. No.: PE-01BHY99)
Related Product 3: CDK1 (6-20) KinSub kinase substrate peptide (Cat. No.: PE-01ADT95)
Related Product 4: Lyn-ANT pan-specific antibody (Cat. No.: AB-NK095-2)
Scientific Background: Lyn is a protein-tyrosine kinase of the TK group and Src family. It is important in signal transduction from cell surface receptors and in the regulation of innate and adaptive immune responses and other cellular functions. Phosphorylation of T503 inhibits its phosphotransferase activity.This kinase is highly expressed and widely distributed in most tested human tissues. Lyn deficiency is involved in defects in some mast cell functions. It interacts with phosphorylated LIME1 and with CD79A upon BCR activation. Lyn is inhibited by phosphorylation at T503. It interacts with a variety of cell surface receptors on the cytoplasmic side of the plasma membrane. Lyn is biosynthetically transported to the plasma membrane via the Golgi pool of caveolin along the secretory pathway that requires its catalytic domain, but not activity. Lyn is expressed preferentially in B cells and can be bound in the plasma membrane with IgM, where it participates in antigen-mediated signal transduction. Crosslinking of membrane-bound IgM with antibody induces rapid increase in activities of Lyn and Lyn-associated PI 3-kinase. Lyn may be involved in the changes that are related to tumour pathogenesis. It is constitutively phosphorylated and activated in cells of chronic myelogenous leukemia (CML) and acute myeloid leukemia (AML) patients. Abnormally enhanced expression levels of activation of Lyn signalling can be critical in survival and proliferation of some types of cancers, inclduing breast carcinomas. This description may include information annotated by UniProt and/or Google AI.