Product Name:
Ltk-pY672
Product Number:
ab-pk687
Target Full Name: Leukocyte tyrosine kinase receptor
Target Alias: Protein tyrosine kinase-1; TYK1; CCDS10077.1; ENSG00000062524
Product Type Specific: LTK protein kinase phosphosite-specific antibody
Antibody Code: PK687
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y672
Protein UniProt: P29376
Protein SigNET: Ltk
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Ltk sequence peptide Cat. No.: PE-04ANO95
Antibody Immunogen Sequence: RDI(pY)RAS(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R669 to S675. Y672 is predicted to be stimulatory for phosphotransferase activity. This phosphosite is located in the kinase activation loop between catalytic subdomains VII and VIII. This is the major in vivo phosphorylation site in LTK (≥24 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Dog | Mouse | Chicken | Zebra fish.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 85-95 kDa.
Related Product 1: LTK (669-675) pY672 antibody blocking peptide (Cat. No.: PE-04ANO95)
Scientific Background: Ltk is a receptor protein-tyrosine kinase of the TK group and Alk family. It is activated by ALKAL1 and ALKAL2 (also known as AUG-alpha-1 or -2) ligands. Studies with chimeric proteins (replacing its extracellular region with that of several known growth factor receptors, such as EGFR and CSFIR) demonstrate its ability to promote melanophore development, growth and specifically neurite outgrowth, and cell survival via activation of the PI3 kinase pathway. It is involved in regulation of cell growth, differentiation, apoptosis and neuronal development via PI3K and MAPK (ERK1/ERK2) patways, and the secretory pathways involving endoplasmic reticulum (ER) export sites (ERESs) and ER to Golgi transport. It is primarily found in hematopoietic cells (specifically B-cell precursors) and neurons. Polymorphisms in the LTK gene are linked to susceptibility to systemic lupus erythematosus (SLE) and abnormal proliferation of B cells. This description may include information annotated by UniProt and/or Google AI. a transmembrane receptor protein belonging to the insulin receptor superfamily that acts as an enzyme to regulate cellular signaling, growth, and differentiation.