Product Name:
LOK-pS191
Product Number:
ab-pk685
Target Full Name: Lymphocyte-oriented protein-serine kinase; Serine-threonine-protein kinase 10
Target Alias: GEK2; STK10; Lymphocyte-oriented kinase; PRO2729
Product Type Specific: LOK protein kinase phosphosite-specific antibody
Antibody Code: PK685
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S191
Protein UniProt: O94804
Protein SigNET: LOK
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human LOK sequence peptide Cat. No.: PE-04AKO99
Antibody Immunogen Sequence: KRD(pS)FIG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues K188 to G194. S191 phosphorylation is predicted to be stimulatory for phosphotransferase activity. This phosphosite is located in the kinase activation loop between catalytic subdomains VII and VIII. This is one of the major in vivo phosphorylation sites in LOK (≥81 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 120-140 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: No significant cross-reactive proteins detected in MCF7 and HeLa cells, except for cross-reactive proteins of ~75 and 90 kDa.
Related Product 1: LOK (188-194) pS191 antibody blocking peptide (Cat. No.: PE-04AKO99)
Related Product 2: IRS1 (979-989) KinSub kinase substrate peptide (Cat. No.: PE-01ADC95)
Related Product 3: LOK-pT952 phosphosite-specific antibody (Cat. No.: AB-PK686)
Scientific Background: LOK (Lymphocyte-Oriented Kinase; STK10) is a 130-138 kDa protein-serine/threonine kinase of the STE group and STE20 family. It can control lymphocyte migration via its phosphotransferase activity. LOK autophosphorylates and phosphorylates meylin basic protein, histone 2A, and MSN (moesin). It is similar to Polo-like kinase kinases and can associate with and phosphorylate PLK1 to affect cell cycle progression. LOK can negatively regulate MEKK1/MAP3K1. It is abundant at the lymphocyte plasma membrane, where it is critical for cytoskeletal rearrangement and other T-cell responses. It features a kinase catalytic domain at the N-terminus (STE20-like) and a long coiled-coil structure at the C-terminus. LOK is highly expressed in lymphocytes, with significant levels in the spleen, thymus, and peripheral blood leukocytes. A K65I mutation in LOK seen with Testicular germ cell tumour (TGCT) can induce loss of kinase phosphotransferase activity. This description may include information annotated by UniProt and/or Google AI.