Product Name:
LOK-pS191
Product Number:
ab-pk685
Target Full Name: Lymphocyte-oriented protein-serine kinase; Serine-threonine-protein kinase 10
Target Alias: GEK2; STK10; Lymphocyte-oriented kinase; PRO2729
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK685
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S191
Protein UniProt: O94804
Protein SigNET: O94804
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human LOK sequence peptide Cat. No.: PE-04AKO99
Antibody Immunogen Sequence: KRD(pS)FIG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues K188 to G194; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues K188 to G194; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 120-140 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: No significant cross-reactive proteins detected in MCF7 and HeLa cells, except for cross-reactive proteins of ~75 and 90 kDa.
Related Product 1: LOK-pS191 blocking peptide
Related Product 2: LOK-pT952 phosphosite-specific antibody (Cat. No.: AB-PK686)
Related Product 3: IRS1 (979-989) KinSub - Insulin receptor substrate 1 (K979-G989, mouse) peptide; Insulin receptor substrate
Scientific Background: LOK (STK10) is a protein-serine/threonine kinase of the STE group and STE20 family. It can control lymphocyte migration via its phosphotransferase activity. LOK has been shown to phosphorylate MSN and has been implicated in phosphorylating Plk1. LOK can negatively regulate MEKK1/MAP3K1, and may also mediate cell cycle progression. A K65I mutation in LOK seen with Testicular germ cell tumour (TGCT) can induce loss of kinase phosphotransferase activity.
