Product Name:
LMR2-pS1450
Product Number:
ab-pk684
Target Full Name: Serine/threonine-protein kinase LMTK2
Target Alias: LMTK2; 2900041G10Rik; AATYK2; BREK; CDK5,p35-regulated kinase; Cprk; KIAA1079; Kinase LMR2; Kinase phosphatase inhibitor 2
Product Type Specific: LMR2 protein kinase phosphosite-specific antibody
Antibody Code: PK684
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S1450
Protein UniProt: Q8IWU2
Protein SigNET: LMR2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human LMR2 sequence peptide Cat. No.: PE-04ANL95
Antibody Immunogen Sequence: KYF(pS)PPP(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues K1447 to P1453; Near the C-terminus of the protein kinase.S1450 phosphorylation stimulates phosphotransferase activity. This is one of the major in vivo phosphorylation sites in LMR2 (≥19 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). LMR2 is known to be phosphorylated at this site in vitro by CDK5.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse | Platypus | Chicken | Zebra fish.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 150-175 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in HeLa and T98G cells, except for a weak cross-reactive protein of ~75 kDa in FBS-treated HeLa cells..
Related Product 1: LMR2 (1447-1453) pS1450 antibody blocking peptide (Cat. No.: PE-04ANL95)
Scientific Background: LMR2 (Lemur Tyrosine Kinase 2; LMTK2) is a 150-175 kDa protein kinase of the TK group and Lmr family. Despite its name, LMTK2 predominantly functions as a serine/threonine-protein kinase, playing a crucial role in regulating various cellular processes including endosomal trafficking, signaling, and neuronal function. It features two N-terminal transmembrane helices and a long C-terminal cytoplasmic tail, acting as a transmembrane protein that allows kinase activity to occur within the cytoplasm. It has an essential role in spermatogenesis. In a large 2-stage genomewide association study of prostate cancer, SNP in intron of the LMR gene is associated susceptibility to prostate cancer. It has been shown to phosphorylate PPP1C, phosphorylase b and CFTR. It is involved in Nerve Growth Factor (NGF)-TrkA signaling and regulates kinesin-1-based axonal transport. It also regulates androgen receptor activity. It is primarily localized to the plasma membrane, with the highest expresssion in skeletal muscle, and lower expression in brain and pancreas. LMTK2 is implicated in Alzheimer's disease, as its loss may affect the axonal transport of p35. It acts as a tumor suppressor in some cancers (e.g., prostate cancer, glioblastoma) and its downregulation is associated with tumor development. Conversely, it acts as a tumor promoter in gastric cancer (GC). This description may include information annotated by UniProt and/or Google AI.