Product Name:
Lck-pY263+pY264
Product Number:
ab-pk680
Target Full Name: Lymphocyte-specific protein-tyrosine kinase; Proto-oncogene tyrosine-protein kinase Lck
Target Alias: LSK; LSK-T; p56-Lck; pp58Lck; RP4-675E8.4; LSK (T-cell specific protein-tyrosine kinase; lck tyrosine kinase; membrane associated protein tyrosine kinase; proto-oncogene Lck; put.ptk; YT16; ENSG00000182866
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK680
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y263+Y264
Protein UniProt: P06239
Protein SigNET: P06239
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Lck sequence peptide Cat. No.: PE-04ARP55
Antibody Immunogen Sequence: GEVWMG(pY)(pY)NGHT(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues G257 to T268; In the kinase catalytic domain between subdomains I and II.
Production Method: Corresponds to amino acid residues G257 to T268; In the kinase catalytic domain between subdomains I and II.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 50-58 kDa.
Related Product 1: Lck-pY263+pY264 blocking peptide
Related Product 2: Lck-pY192 phosphosite-specific antibody (Cat. No.: AB-PK679)
Related Product 3: LckSubtide - Lck protein kinase substrate peptide
Scientific Background: Lck is a non-receptor protein-tyrosine kinase of the TK group and Src family. Phosphorylation of Y394 increases phosphotransferase activity and induces interaction with Lck. Phosphorylation of Y192 and Y505 inhibits Lck phosphotransferase activity. Lck has a critical function in the selection and maturation of developing T-cells in the thymus, as well as in the function of mature T-cells throughout the body. Lck is a key signalling component of the T-cell antigen receptor (TCR)-linked signal transduction pathway and is constitutively bound to the cytoplasmic domains of the CD4 and CD8 membrane receptors. Lck is expressed through all stages of thymocyte development and is necessary for regulating both pre-TCR and mature alpha beta TCR-mediated maturation. Lck may be an oncoprotein (OP) based on its similarity to other Src family protein-tyrosine kinases.
