Product Name:
Lck-pY192
Product Number:
ab-pk679
Target Full Name: Lymphocyte-specific protein-tyrosine kinase; Proto-oncogene tyrosine-protein kinase LCK
Target Alias: LSK; LSK-T; p56-Lck; pp58Lck; RP4-675E8.4; LSK (T-cell specific protein-tyrosine kinase; lck tyrosine kinase; membrane associated protein tyrosine kinase; proto-oncogene Lck; put.ptk; YT16; ENSG00000182866
Product Type Specific: Lck protein kinase phosphosite-specific antibody
Antibody Code: PK679
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y192
Protein UniProt: P06239
Protein SigNET: Lck
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Lck sequence peptide Cat. No.: PE-04AKN95
Antibody Immunogen Sequence: GGF(pY)ISP(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues G189 to P195; in the SH2 domain. Y192 phosphorylation inhibits phosphotransferase activity. Phosphorylation regulates molecular association. This is one of the major in vivo phosphorylation sites in Lck (≥1092 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very weak immunoreactivity of a target-sized protein by Western blotting in Jurkat cells. Strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Dog | Rat | Mouse | Platypus | Chicken.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 50-58 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactivities in HeLa and Jurkat cells.
Related Product 1: Lck (189-195) pY192 antibody blocking peptide (Cat. No.: PE-04AKN95)
Related Product 2: LckSubtide kinase substrate peptide (Cat. No.: PE-01BHV90)
Related Product 3: Lck-pY263+pY264 phosphosite-specific antibody (Cat. No.: AB-PK680)
Scientific Background: Lck (lymphocyte-specific protein tyrosine kinase) is a 56 kDa non-receptor protein-tyrosine kinase of the TK group and Src family. It features an SH4 domain (for membrane localization), SH3 and SH2 domains (protein interaction), a kinase domain, and a C-terminal inhibitory tail. Its activity is regulated by phosphorylation at its C-terminal tail by Csk (inhibitory) and dephosphorylation by CD45 (activating). Phosphorylation of Y394 increases phosphotransferase activity and induces interaction with Lck. It is crucial for activating T-cells by phosphorylating immunoreceptor tyrosine-based activation motifs (ITAMs). Phosphorylation of Tyr-192 and Tyr-505 inhibits Lck phosphotransferase activity. Lck has a critical function in the selection and maturation of developing T-cells in the thymus, as well as in the function of mature T-cells throughout the body. Lck is a key signalling component of the T-cell antigen receptor (TCR)-linked signal transduction pathway and is constitutively bound to the cytoplasmic domains of the CD4 and CD8 membrane receptors and facilitates the activation of ZAP-70 upon T-cell antigen receptor stimulation. Lck is primarily expressed in T-cells through all stages of thymocyte development and is necessary for regulating both pre-TCR and mature alpha beta TCR-mediated maturation. Lck may be an oncoprotein (OP) based on its similarity to other Src family protein-tyrosine kinases. Mutations or dysfunction are linked to Severe Combined Immunodeficiency (SCID). Overexpression is associated with autoimmune conditions such as rheumatoid arthritis, asthma, and lupus. Overexpression of Lck also drives tumorigenesis in thymomas and is found in some colorectal cancers. This description may include information annotated by UniProt and/or Google AI.