Product Name:
Ksr1-pS406
Product Number:
ab-pk675
Target Full Name: Protein-serine kinase suppressor of Ras 1
Target Alias: CAP kinase; Ceramide-activated protein kinase; HB; HB protein; Ksr; Ksr1; Kinase suppressor of ras 1; Kinase suppressor of RAS-1; RSU2; ENSG00000141068
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK675
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S406
Protein UniProt: Q8IVT5
Protein SigNET: Q8IVT5
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Ksr1 sequence peptide Cat. No.: PE-04ANE99
Antibody Immunogen Sequence: RTE(pS)VPS(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R401 to S407; In the region between the Ksr1-SAM and kinase catalytic domains. This is the major in vivo phosphorylation site in Krs1.
Production Method: Corresponds to amino acid residues R401 to S407; In the region between the Ksr1-SAM and kinase catalytic domains. This is the major in vivo phosphorylation site in Krs1.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 105-120 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: No significant cross-reactivities in HepG2, Jurkat and T98G cells and in sea star oocytes, except for a ~65 kDa protein in HepG2 and T98G cells, and a strong 50 kDa protein in sea star oocytes.
Related Product 1: Ksr1-pS406 blocking peptide
Related Product 2: Ksr1 pan-specific antibody (Cat. No.: AB-NK090)
Related Product 3: Ksr2-pS490 phosphosite-specific antibody (Cat. No.: AB-PK676)
Scientific Background: Ksr1 (kinase suppressor of Ras 1) is a protein-serine/threonine kinase of the TKL group and RAF family. It is a Raf-related pseudokinase and may directly mediate Raf activation. It acts as a scaffold for the Raf/MEK/ERK kinase cascade. No specific mutations have been directly implicated in oncogenesis although loss of Ksr1 function will blunt the Ras pathway and therefore attenuate its oncogenic potential.
