Product Name:
Kit -S821+Y823
Product Number:
ab-pk673
Target Full Name: Mast/stem cell growth factor receptor protein-tyrosine Kit
Target Alias: CD117; C-kit; Kinase Kit; Mast/stem cell growth factor receptor precursor; PBT; SCFR; SL; V-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene; V-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homologue; ENSG00000157404
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK674
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S821+Y823
Protein UniProt: P10721
Protein SigNET: P10721
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Kit sequence peptide Cat. No.: PE-04ARM95
Antibody Immunogen Sequence: KND(pS)N(pY)VVKG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues K818 to G827; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues K818 to G827; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in Jurkat cells. Strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 125-145 kDa.
Antibody Specificity: Low
Antibody Cross Reactivity: High cross-reactivity in Jurkat cells, including phenylarsine oxide (PAO) increase in 15 kDa protein. Backgroud was also rather high with this antibody.
Related Product 1: Kit -S821+Y823 blocking peptide
Related Product 2: Kit-1 pan-specific antibody (Cat. No.: AB-NK241-1)
Related Product 3: Kit-2 pan-specific antibody (Cat. No.: AB-NK241-2)
Related Product 4: Kit-pY936 phosphosite-specific antibody (Cat. No.: AB-PK673)
Scientific Background: Kit (SCFR) is a protein-tyrosine kinase of the TK group and PDGFR family. It is a receptor kinase that binds SCF/KitLG. Mast,stem cell growth factor (steel factor) binding induces Kit dimerization and autophosphorylation. Protein interactions are induced with phosphorylation at Y568 (with APS, Cbl, SHP2, & Src), Y900 (with Crk & PIK3C2A) and Y936 (with APS, Cbl, Grb2 & Grb7). Phosphorylation of S741 and S746 inhibits the kinase activity of Kit. Kit can mediate cell proliferation, survival, hematopoiesis, gametogenesis, stem cell maintenance, mast cell development, function, and migration, as well as melanogenesis. Some substrates that Kit can phosphorylate include PIK3R1, PLCG1, SH2B2/APS and CBL.This kinase is highly expressed and widely distributed in most tested human tissues. It was first identified as the cellular homologue of the feline sarcoma viral oncogene v-kit. Mutations in Kit are associated with gastrointestinal stromal tumors (GIST), mast cell leukemia, acute myelogenous leukemia (AML), and piebaldism. Kit has also been linked with the development of colorectal adenocarcinomas and melanoma progression. Insertional mutagenesis studies in mice also support a role for this protein kinase in mouse cancer oncogenesis.
