Product Name:
KHS1-pS174
Product Number:
ab-pk671
Target Full Name: Kinase homologous to SPS1/STE20 (MAP kinase kinase kinase protein-serine kinase 5 (MEKKK5)
Target Alias: GCKR; Germinal center kinase-related; Germinal center kinase-related enzyme; KHS; Kinase KHS1; M4K5; MAP4K5; MAPKKKK5; Q9Y4K4; ENSG00000012983
Product Type Specific: KHS1 (MAP4K5) protein kinase phosphosite-specific antibody
Antibody Code: PK671
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S174
Protein UniProt: Q9Y4K4
Protein SigNET: KHS1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human KHS1 (MAP4K5; KHS) sequence peptide Cat. No.: PE-04AND99
Antibody Immunogen Sequence: KRK(pS)FIG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues K171 to G177; in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. S174 phosphorylation stimulates phosphotransferase activity. This is one of the minor in vivo phosphorylation sites in KHS1 (≥6 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in sea star oocytes. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse | Platypus.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 85-100 kDa.
Antibody Specificity: Medium
Antibody Cross Reactivity: Many cross-reactive proteins detected in sea star oocytes, including a strongly immunoreactive 150 kDa protein.
Related Product 1: KHS1 (171-177) pS174 antibody blocking peptide (Cat. No.: PE-04AND99)
Related Product 2: KHS1-ANT pan-specific antibody (Cat. No.: AB-NK089-2)
Scientific Background: KHS1 (MAP4K5; CCKR) is a protein-serine/threonine kinase of the STE group and STE20 family. It has been proposed to be upstream of the JUN N-terminal pathway and may respond to environmental stresses. It features a conserved N-terminal kinase domain and a large C-terminal regulatory domain, with a molecular mass of approximately 135 kDa. KHS1 interacts with adapter proteins like Crk and CrkL. A mutation in KHS1, K49R, inhibits the kinase phosphotransferase activity and inhibits downstream JNK proteins. It is involved in TNF-alpha-induced signaling in lymphocytes, such as Jurkat T cells. KHS1 links the Bcr-Abl oncogene and Ras to the stress-activated protein kinase pathway. It is ubiquitously expressed, with higher levels reported in the testis, ovary, and prostate. It is primarily found in the cytoplasm, with some localization to the plasma membrane and primary. KHS1 has been linked to diseases like Spastic Paraplegia 3A and Septal Myocardial Infarction. It may play a role in inflammatory responses, including obesity and type 2 diabetes. This description may include information annotated by UniProt and/or Google AI.