Product Name:
JNK1-pY185
Product Number:
ab-pk670
Target Full Name: Jun N-terminus protein-serine kinase (stress-activated protein kinase (SAPK)) 1
Target Alias: c-Jun N-terminal kinase 1; JNK; JNK1A2; JNK1-alpha-2; JNK-46;JNK21B1/2; JUN N-terminal kinase; Kinase JNK1; MAPK8; MK08; PRKM8; SAPK1; SAPK1c; Stress-activated protein kinase JNK1; CCDS7225.1; ENSG00000107643
Product Type Specific: JNK1 (MAPK8) protein kinase phosphosite-specific antibody
Antibody Code: PK670
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y185
Protein UniProt: P45983
Protein SigNET: JNK1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human JNK1 (MAPK8) sequence peptide Cat. No.: PE-04AEM95
Antibody Immunogen Sequence: MTP(pY)VVT(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues M182 to T188. Y185 phosphorylation stimulates phosphotransferase activity. This phosphosite is located in the kinase activation loop between catalytic subdomains VII and VIII. This is one of the major in vivo phosphorylation sites in JNK1 (≥1286 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). JNK1 is known to be phosphorylated at this site in vitro by ASK1 (MAP3K5), MEKK1 (MAP3K1), MEKK6 (MAP3K6, ASK2), MKK4 (MAP2K4, MEK4), and MKK7 (MAP2K7, MEK7).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots. Medium-strong immunoreactivity with recombinant human JNK1 on protein dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Dog | Rat | Mouse | Platypus | Frog.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 43-46 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: Strong immunoreactivity on protein dot blots with recombinant human JNK2, but very weak immunoreactivity with JNK3. No significant cross-reactive proteins detected in A431, A549, HeLa and MCF7 cells and in sea star oocytes, except for a ~77 kDa cross-reactive protein in A431 and Hela cells.
Related Product 1: JNK1 (182-188) pY185 antibody blocking peptide (Cat. No.: PE-04AEM95)
Related Product 2: JNKSelectideA kinase substrate peptide (Cat. No.: PE-14CAD90)
Related Product 3: JNKSubtide kinase substrate peptide (Cat. No.: PE-01BHT95)
Related Product 4: JNK1 expression antibody (Cat. No.: AB-NK217-2)
Related Product 5: JNK1 expression antibody (Cat. No.: AB-NK217-3)
Related Product 6: JNK1 T183 phosphorylation antibody (Cat. No.: AB-PK969)
Related Product 7: JNK2 T183+Y185 phosphorylation antibody (Cat. No.: AB-PK902)
Related Product 8: JNK2 expression antibody (Cat. No.: AB-NK189-2)
Related Product 9: JNK3 expression antibody (Cat. No.: AB-NK197)
Related Product 10: JNK3 expression antibody (Cat. No.: AB-NK197-2)
Scientific Background: JNK1 (MAPK8) is a protein-serine/threonine kinase of the CMGC group and MAPK family. It is involved in the regulation of several cellular processes, including cell proliferation, differentiation, migration, transformation, and apoptosis, through the JNK/SAP signalling pathway. This pathway is activated by extracellular stimuli such as pro-inflammatory cytokines or physical stress, as well as signals of intracellular stress, such as misfolded proteins in the endoplasmic reticulum. It is also activated by high fat diet in fat, muscle, and liver tissues, leading to increased phosphorylation of IRS1 and contributing to insulin resistance. Phosphorylation of Thr-183 and Tyr-185 by either of two dual specificity kinases, MAP2K4 and MAP2K7, increases phosphotransferase activity. It is inhibited by dual specificity phosphatases, such as DUSP1, which target the T183 and Y185 activatory phosphorylation sites. It promotes apoptosis (cell death) by upregulating pro-apoptotic genes, but can also promote survival in specific contexts. Apart from c-Jun, substrates include proteins involved in T-cell differentiation, microtubule dynamics (via STMN2 phosphorylation), and the circadian clock. JNK1 is associated with cancer pathogenesis, including liver and colorectal cancers, as well as obesity-induced inflammation. This description may include information annotated by UniProt and/or Google AI.