Product Name:
KHS1-pS174
Product Number:
ab-pk670
Target Full Name: Kinase homologous to SPS1/STE20 (MAP kinase kinase kinase protein-serine kinase 5 (MEKKK5)
Target Alias: GCKR; Germinal center kinase-related; Germinal center kinase-related enzyme; KHS; Kinase KHS1; M4K5; MAP4K5; MAPKKKK5; Q9Y4K4; ENSG00000012983
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK671
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S174
Protein UniProt: Q9Y4K4
Protein SigNET: Q9Y4K4
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human KHS1 (MAP4K5; KHS) sequence peptide Cat. No.: PE-04AND99
Antibody Immunogen Sequence: KRK(pS)FIG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues K171 to G177; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues K171 to G177; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in sea star oocytes. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 85-100 kDa.
Antibody Specificity: Medium
Antibody Cross Reactivity: Many cross-reactive proteins detected in sea star oocytes, including a strongly immunoreactive 150 kDa protein.
Related Product 1: KHS1-pS174 blocking peptide
Related Product 2: KHS1-ANT pan-specific antibody (Cat. No.: AB-NK089-2)
Scientific Background: KHS1 (MAP4K5) is a protein-serine/threonine kinase of the STE group and STE20 family. It has been proposed to be upstream of the JUN N-terminal pathway and may respond to environmental stresses. A mutation in KHS1, K49R, inhibits the kinase phosphotransferase activity and inhibits downstream JNK proteins.
