Product Name:
JNK1-pY185
Product Number:
ab-pk669
Target Full Name: Jun N-terminus protein-serine kinase (stress-activated protein kinase (SAPK)) 1
Target Alias: c-Jun N-terminal kinase 1; JNK; JNK1A2; JNK1-alpha-2; JNK-46;JNK21B1/2; JUN N-terminal kinase; Kinase JNK1; MAPK8; MK08; PRKM8; SAPK1; SAPK1c; Stress-activated protein kinase JNK1; CCDS7225.1; ENSG00000107643
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK670
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y185
Protein UniProt: P45983
Protein SigNET: P45983
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human JNK1 (MAPK8) sequence peptide Cat. No.: PE-04AEM95
Antibody Immunogen Sequence: MTP(pY)VVT(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues M182 to T188; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues M182 to T188; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots. Medium-strong immunoreactivity with recombinant human JNK1 on protein dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 43-46 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: Strong immunoreactivity on protein dot blots with recombinant human JNK2, but very weak immunoreactivity with JNK3. No significant cross-reactive proteins detected in A431, A549, HeLa and MCF7 cells and in sea star oocytes, except for a ~77 kDa cross-reactive protein in A431 and Hela cells.
Related Product 1: JNK1-pY185 blocking peptide
Related Product 2: JNK1-1 pan-specific antibody (Cat. No.: AB-NK217-2)
Related Product 3: JNK1-2 pan-specific antibody (Cat. No.: AB-NK217-3)
Related Product 4: JNK2-1 pan-specific antibody (Cat. No.: AB-NK189-2)
Related Product 5: JNK2-3 pan-specific antibody (Cat. No.: AB-NK189-4)
Related Product 6: SAPKa/b (JNK2-4) pan-specific antibody (Cat. No.: AB-NK196)
Related Product 7: JNK3-1 pan-specific antibody (Cat. No.: AB-NK197-2)
Related Product 8: JNK3-3 pan-specific antibody (Cat. No.: AB-NK197-4)
Related Product 9: JNKSelectideA - JNK1 protein kinase substrate peptide
Related Product 10: JNKSubtide - JNK1 (MAPK8) protein kinase substrate peptide
Scientific Background: JNK1 (MAPK8) is a protein-serine/threonine kinase of the CMGC group and MAPK family. It is involved in the regulation of several cellular processes, including cell proliferation, differentiation, migration, transformation, and apoptosis, through the JNK/SAP signalling pathway. This pathway is activated by extracellular stimuli such as pro-inflammatory cytokines or physical stress, as well as signals of intracellular stress, such as misfolded proteins in the endoplasmic reticulum. Phosphorylation of T183 and Y185 by either of two dual specificity kinases, MAP2K4 and MAP2K7, increases phosphotransferase activity. It is inhibited by dual specificity phosphatases, such as DUSP1, which target the T183 and Y185 activatory phosphorylation sites.
