Product Name:
ITK-pY512
Product Number:
ab-pk665
Target Full Name: Tyrosine-protein kinase ITK/TSK
Target Alias: EMT; Lyk; PSCTK2; MGC126257; MGC126258; TLK; TSK; CCDS4336.1; ENSG00000113263
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK666
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y512
Protein UniProt: Q08881
Protein SigNET: Q08881
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human ITK sequence peptide Cat. No.: PE-04ANB90
Antibody Immunogen Sequence: DDQ(pY)TSS(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues D509 to S515; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues D509 to S515; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in Jurkat cells. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 68-72 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: No significant cross-reactivities detected in Jurkat cells, except phenylarsine oxide (PAO) increases 15 kDa protein immunoreactivity.
Related Product 1: ITK-pY512 blocking peptide
Related Product 2: ITK-BCT pan-specific antibody (Cat. No.: AB-NK274-1)
Scientific Background: ITK is a protein-serine/threonine kinase that is a member of the TK group of protein kinases in the Tec family. This kinase is widely distributed from moderate to high levels in most human tissues with highest expression in bladder, lung, lymph node, spleen, stomach, thymus, tonsils and uterus. Ligation of CD2, TCR or CD28 induces activation and tyrosine phosphorylation of ITK at Y512. ITK is expressed in T-cells and is important for T-cell development and activation through the antigen receptor. ITK require prior activation of Lck, ZAP70 and PI3-kinase for efficient activation. ITK knockout mice show multiple effects on T cell development, cytokine production and T-helper cell differentiation. T cells that lack or express mutant versions of ITK show impaired TCR-induced actin polymerization, cell polarization and regulation of the signalling events involved in cytoskeletal reorganization. ITK has been linked with the development of gastric adenocarcinomas, melanoma (metastatic) and lymphoproliferative syndrome ebv-associated autosomal type 1 (LPSA1).
