Product Name:
IKKe-pS172
Product Number:
ab-pk660
Target Full Name: Inhibitor of nuclear factor kappa-B kinase epsilon
Target Alias: I kappa-B kinase epsilon; IkBKE; IKKE; IKK-E; IKK-i; IKKI; KIAA0151; RP11-534L20.1; KIAA0151; MGC125294; MGC125295; MGC125297; ENSG00000143466
Product Type Specific: IKKe protein kinase phosphosite-specific antibody
Antibody Code: PK660
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S172
Protein UniProt: Q14164
Protein SigNET: IKKe
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human IKKe (IkBKE) sequence peptide Cat. No.: PE-04AMW99
Antibody Immunogen Sequence: KFV(pS)VYG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues K169 to G175; in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. S172 phosphorylation stimulates phosphotransferase activity. This is one of the less prominant in vivo phosphorylation sites in IKKe (≥4 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). IKKe is known to be phosphorylated at this site in vitro by IKKe (IKBKE).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in MCF7 cells and sea star oocytes. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Dog | Mouse | Platypus | Frog | Honey bee.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 80-87 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in MCF7 and T98G cells.
Related Product 1: IKKe (169-175) pS172 antibody blocking peptide (Cat. No.: PE-04AMW99)
Related Product 2: IKKSelectide kinase substrate peptide (Cat. No.: PE-01BHN95)
Related Product 3: IKKa-pT179+pS180 phosphosite-specific antibody (Cat. No.: AB-PK659)
Related Product 4: IKKb-BCT pan-specific antibody (Cat. No.: AB-NK076-5)
Related Product 5: IKKb-NT pan-specific antibody (Cat. No.: AB-NK076-6)
Related Product 6: IKKg S377 phosphorylation antibody (Cat. No.: AB-PN758)
Scientific Background: IKKe (IKBKE) is an 80 kDa protein-serine/threonine kinase of the Other group and IKK family. It mediates inflammatory responses in response to viral infections. It is activated by RIG-I-like receptors, TNFa, and IL-1, it operates through various scaffolding molecules like MAVS, TANK, and SINTBAD. It features an N-terminal kinase domain and a C-terminal ubiquitin-like domain (ULDBP3D) that aids in scaffolding. While it shares structural homology with canonical IKKs (alpha/ and beta), IKKe functions independently of the IKKg (NEMO) regulatory subunit. Phosphorylation of IKKe at Ser-172 increases phosphotransferase activity. Ubiquitination and decreased kinase phosphotransferase activity stems from a K30A, or K401A, or K30R, or K401R mutation in IKKe. K30R and K401R will additionally reduce interactions with IKKb, IKBKG, and MyD88. The K38A mutation leads to kinase phosphotransferase activity inhibition and inhibited nuclear transport. Sumoylation and targeting to nuclear bodies is lost with a K231R mutation to IKKe. It is heavily involved in NF-κB activation and inflammatory responses. IKKe regulates inflammatory responses by acting as a sensor for viral RNA, often working with adaptor proteins like MAVS, TANK, or SINTBAD. IKKe is primarily expressed in the thymus, spleen, and peripheral blood leukocytes, reflecting its strong role in immune cell regulation. IKKε is implicated in maintaining low-grade inflammation in obesity and impacting insulin sensitivity. It acts as an oncogene in certain cancers. For example, IKKε is overexpressed in over 30% of breast cancers, promoting cell transformation and proliferation. This description may include information annotated by UniProt and/or Google AI.