Product Name:
IGF1R-pY1346
Product Number:
ab-pk658
Target Full Name: Insulin-like growth factor 1 receptor
Target Alias: CD221; IGFIR; Insulin-like growth factor I receptor; JTK13; MGC142170; MGC142172
Product Type Specific: IGF1R protein kinase phosphosite-specific antibody
Antibody Code: PK658
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y1346
Protein UniProt: P08069
Protein SigNET: IGF1R
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human IGF1R sequence peptide Cat. No.: PE-04AKL99
Antibody Immunogen Sequence: RQP(pY)AHM(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R1343 to M1349; in the C-terminal of the kinase after the catalytic domain. Y1346 phosphorylation stimulates phosphotransferase activity and induces binding to Grb10. Phosphorylation regulates cell differentiation, cell growth, molecular association and protein interaction. This is one of the minor in vivo phosphorylation sites in IGF1R based on ≥34 high throughput mass spectrometry reports recorded in PhosphoSitePlus. IGF1R is known to be phosphorylated at this site in vitro by IGF1R, and Src.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Frog | Zebra fish.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 90-100, 130 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in A431, HEK-293 and MCF7 cells, and in sea star oocytes.
Related Product 1: IGF1R (1343-1349) pY1346 antibody blocking peptide (Cat. No.: PE-04AKL99)
Related Product 2: IGF1R-BKCD pan-specific antibody (Cat. No.: AB-NK074-2)
Related Product 3: IGF1R-CT pan-specific antibody (Cat. No.: AB-NK074-3)
Related Product 4: IGF1R-pY1161+pT1163 phosphosite-specific antibody (Cat. No.: AB-PK657)
Scientific Background: IGF1R is a ~155 KDa protein-tyrosine kinase of the TK group and InsR family. It mediates the effects of its ligand insulin-like growth factor 1 (IGF1) to promote cell growth and survival. The pre-dimerized receptor also displays weak affinity for both insulin and IGF2. Once ligand bound, the IGF1R undergoes autophosphorylation and activates several downstream signalling pathways, including the PI3K-Akt pathway and the Ras/Raf/MAP kinase pathway to induce cell growth and survival. Autophosphorylation Tyr-1161 and Tyr-346 increases its phosphotransferase activity and induces interaction with Src. Tyr-1346 autophosphorylation also mediates GRB10 binding to IGF1R. Tyr-980, Tyr-1161, Tyr-1165 and Tyr-1166 autophosphorylation also increases its phosphotransferase activity. Tyr-980 autophosphorylation also appears to mediate IRS1 or Shc1 interactions with IGF1R. IGFR1 acts as an oncoprotein (OP). Cancer-related mutations in human tumours point to a gain of function of the protein kinase. The active form of the protein kinase normally acts to promote tumour cell proliferation. IGF1R been shown to be an important signalling component in several types of human cancers. In particular, the activated receptor is critical for tumour transformation, malignant cell survival, and the maintenance of the transformed phenotype. In addition, activated IGF1R protein functions to inhibit apoptosis via indirect phosphorylation of the BAD protein via Akt, as well as plays an important role in the regulation of cancer cell motility and metastasis. Several cancer types display increased expression of IGF1R and/or increased catalytic activity of the protein, including prostate cancer, colorectal adenoma, and melanoma. Additionally, cross-talk between the IGF signalling pathway and the estrogen receptor signalling pathway has been demonstrated in breast cancer cells as a central mechanism of cell growth regulation. Therapies aimed at the inhibition of IGF1R signalling have shown promise as potential cancer treatments. Insertional mutagenesis studies in mice support a role for this protein kinase in mouse cancer oncogenesis. This description may include information annotated by UniProt and/or Google AI.