Product Name:
ICK-pY159
Product Number:
ab-pk656
Target Full Name: Intestinal cell (MAK-like) kinase
Target Alias: ECO; HICK; Intestinal cell (MAK-like) kinase; Intestinal cell kinase; KIAA0936; Kinase ICK; Laryngeal cancer kinase 2; LCK2; MAK-related kinase; MGC46090; MRK; Serine/threonine kinase ICK
Product Type Specific: ICK protein kinase phosphosite-specific antibody
Antibody Code: PK656
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y159
Protein UniProt: Q9UPZ9
Protein SigNET: ICK
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human ICK sequence peptide
Antibody Immunogen Sequence: YTD(pY)VST(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues Y156 to T162. Y159 phosphorylation stimulates phosphotransferase activity. This phosphosite is located in the kinase activation loop between catalytic subdomains VII and VIII. This is one of the major in vivo phosphorylation sites in ICK (≥448 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). ICK is known to be phosphorylated at this site in vitro by ICK.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Platypus | Zebra fish.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 64-67 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in MCF7 cells, except for 2 low molecular mass proteins. In maturing sea star oocytes, the induction of strong phosphorylation of a 30 kDa cross-reactive protein was observed.
Related Product 1: ICK expression antibody (Cat. No.: AB-NK073-3)
Related Product 2: ICK expression antibody (Cat. No.: AB-NK073-4)
Related Product 3: ICK expression antibody (Cat. No.: AB-NK073-5)
Related Product 4: ICK Y156+T157 phosphorylation antibody (Cat. No.: AB-PK655)
Scientific Background: ICK (Intestinal cell kinase; CILK1; Ciliogenesis Associated Kinase-1) is a protein-serine/threonine kinase of the CMGC group and RCK family. ICK functions in the regulation of proliferation and differentiation in intestinal cells. It displays sequence similarity to the MAP kinases in the catalytic domain and the requirement for dual phosphorylation for activation within a MAP kinase-like TDY motif. It also features an intrinsically disordered C-terminal domain (CTD), which allows ICK to localize to the base and tip of primary cilia, with its C-terminal domain required for this localization and for interaction with the IFT-B complex. It acts as a key regulator of ciliary length, Hedgehog signaling, and cell cycle progression. It plays a crucial role in ciliogenesis (forming primary cilia) and acts as a kinase for proteins like KIF3A, which regulates cilia transport. ICK homologues in algae and invertebrates function in the formation of flagella and in ciliogenesis. Impairing ICK function has been shown to down-regulate the translation of specific targets of eIF4E and S6K1 (e.g., cyclin D1 and c-Myc) mediated through the mTOR/Raptor signalling pathway. ICK interacts with the mTOR/Raptor complex and phosphorylates Raptor. It is widely expressed across tissues, with high levels in the testis and placenta. Mutations in the gene are associated with endocrine-cerebro-osteodysplasia syndrome (ECO syndrome) and cranioectodermal dysplasia. ICK is elevated in some primary colon cancer specimens and may contribute to malignant proliferation. This description may include information annotated by UniProt and/or Google AI.