Product Name:
ICK-pY159
Product Number:
ab-pk655
Target Full Name: Intestinal cell (MAK-like) kinase
Target Alias: ECO; HICK; Intestinal cell (MAK-like) kinase; Intestinal cell kinase; KIAA0936; Kinase ICK; Laryngeal cancer kinase 2; LCK2; MAK-related kinase; MGC46090; MRK; Serine/threonine kinase ICK
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK656
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y159
Protein UniProt: Q9UPZ9
Protein SigNET: Q9UPZ9
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human ICK sequence peptide
Antibody Immunogen Sequence: YTD(pY)VST(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues Y156 to T162; In the protein kinase catalytic domain in subdomain I.
Production Method: Corresponds to amino acid residues Y156 to T162; In the protein kinase catalytic domain in subdomain I.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 64-67 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in MCF7 cells, except for 2 low molecular mass proteins. In maturing sea star oocytes, the induction of strong phosphorylation of a 30 kDa cross-reactive protein was observed.
Related Product 1: ICK-pY159 phosphosite-specific antibody (Cat. No.: AB-PK656)
Scientific Background: ICK is a protein-serine/threonine kinase of the CMGC group and RCK family. It displays sequence similarity to the MAP kinases in the catalytic domain and the requirement for dual phosphorylation for activation within a MAP kinase-like TDY motif. ICK functions in the regulation of proliferation and differentiation in intestinal cells. Impairing ICK function has been shown to dowregulate the translation of specific targets of eIF4E and S6K1 (e.g. cyclin D1 and c-Myc) mediated through the mTOR/Raptor signalling pathway. Additionally, ICK homologuess in algae and invertebrates function in the formation of flagella and in ciliogenesis.
