Product Name:
HIPK1-pY352
Product Number:
ab-pk654
Target Full Name: Homeodomain-interacting protein-serine kinase 1
Target Alias: HIK1; Homeodomain interacting protein kinase 1; KIAA0630; MGC26642; MGC33446; MGC33548; Myak; Nbak2
Product Type Specific: HIPK1 protein kinase phosphosite-specific antibody
Antibody Code: PK654
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y352
Protein UniProt: Q86Z02
Protein SigNET: HIPK1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human HIPK1 sequence peptide Cat. No.: PE-04AKK95
Antibody Immunogen Sequence: CST(pY)LQS(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues C349 to S355. Y352 phosphorylation is predicted to be stimulatory for phosphotransferase activity. This phosphosite is located in the kinase activation loop between catalytic subdomains VII and VIII. This is the major in vivo phosphorylation site in HIPK1 (≥3394 reports from high throughput mass spectrometry studies reported in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity of a target-sized protein by Western blotting in maturing, germinal vesicle positive, sea star oocytes. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 125-135 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: No significant cross-reactive proteins detected in T98G cells. Strong 40 and 46 kDa cross-reactive proteins were detected in MCF7 cells and strong 22 kDa cross-reactive proteins in HeLa cells.
Related Product 1: HIPK1 (349-355) pY352 antibody blocking peptide (Cat. No.: PE-04AKK95)
Related Product 2: HIPK2 expression antibody (Cat. No.: AB-NK272-1)
Related Product 3: DYRKtide KinSub kinase substrate peptide (Cat. No.: PE-01ACQ95)
Scientific Background: HIPK1 (HIK1) is a protein-serine/threonine kinase of the CMGC group and DYRK family. It is a regulator of cell proliferation, apoptosis, and differentiation, and development, specifically in the eye and angiogenesis. It functions in transcription regulation as well as tumour necrosis factor (TNF)-mediated apoptosis. It resides mainly in the nucleus, controlling transcription, but translocates to the cytoplasm under stress (e.g., TNF-alpha). HIPK1 is also known to act as a co-repressor for homeodomain transcription factors. When present, TNF induces nuclear translocation of HIPK1, thus removing it from the cytoplasm. While predominantly nuclear, it can relocate to the cytoplasm via SENP1-mediated desumoylation during stress to activate MAP3K5-JNK signaling. In addition, through the direct phosphorylation of p53 at the Ser-15 residue, HIPK1 activates p53 leading to increased p21 protein levels, which subsequently inhibits cell proliferation. Other substrates include DAXX, Myb and PAGE. Under stress, HIPK1 phosphorylates DAXX and facilitates its translocation from the nucleus to the cytoplasm, promoting apoptosis. It acts as a positive regulator of JNK signaling. Its activity is regulated by SUMOylation, and its stability can be influenced by O-GlcNAcylation. It is highly expressed in hematopoietic cells (B and T cells) and various tissues, including brain regions. HIPK1 may be a tumour suppressor protein (TSP). Over-expression of HIPK1 has been observed in colorectal cancer specimens, with highest levels at the beginning of tumorigenesis followed by a progressive reduction over time as the cancer develops, indicating a role for the HIPK1 protein in the early events of tumorigenesis. In addition, these specimens also displayed native p53 and normal levels of p21, indicating that the HIPK1 protein functions as a tumour suppressor and that a loss-of-function in the protein is associated with tumorigenesis. This description may include information annotated by UniProt and/or Google AI.