Product Name:
HGK-pT187
Product Number:
ab-pk653
Target Full Name: Mitogen-activated protein kinase kinase kinase kinase 4
Target Alias: FLH21957; Hepatocyte progenitor kinase-like/germinal center kinase-like kinase; HGK; HPK/GCK-like kinase HGK; M4K4; MAP4K4; MAPK kinase kinase kinase 4; MAPK/ERK kinase kinase kinase 4; MEK kinase kinase 4; MEKKK 4; MEKKK4; Mitogen-activated protein kinase kinase kinase kinase 4; Nck interacting kinase; NIK; FLJ10410; FLJ20373; FLJ90111; KIAA0687; NIK; O95819; ENSG0000007105
Product Type Specific: HGK (MAP4K4) protein kinase phosphosite-specific antibody
Antibody Code: PK653
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T187
Protein UniProt: O95819
Protein SigNET: HGK
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human HGK (ZC1) sequence peptide Cat. No.: PE-04ALN99
Antibody Immunogen Sequence: RRN(pT)FIG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R184 to G190. T187 phosphorylation stimulates HGK phosphotransferase activity. This phosphosite is located in the kinase activation loop between catalytic subdomains VII and VIII. This is one of the major in vivo phosphorylation sites in HGK (≥110 reports from high throughput mass spectrometry studies in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in serum-starved Jurkat cells treated with insulin for 5 minutes. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 135-150 kDa.
Antibody Specificity: High-very high
Related Product 1: HGK (178-190) pT181+pT187 antibody blocking peptide (Cat. No.: PE-04AXI99)
Related Product 2: HGK expression antibody (Cat. No.: AB-NK300-1)
Scientific Background: ZC1 (Hepatocyte progenitor kinase-like/Germinal center kinase-like Kinase; HGK; MAP4K4) is a protein-serine/threonine kinase of the STE group and STE20 family. It may play a role in the response to environmental stress and cytokines such as TNF-alpha, and appears to act upstream of the JUN N-terminal pathway. ZC1 specifically activates the MAPK8/JNK signaling pathway, frequently functioning through the TAK1-MKK4/MKK7 kinase cascade. it is involved in regulating actin cytoskeletal organization, inhibiting adipogenesis (fat cell formation), and mediating TNF-alpha-induced insulin resistance. It features an N-terminal kinase domain, a coiled-coil domain, and a C-terminal hydrophobic leucine-rich citron homology (CNH) domain, which is crucial for protein-protein interactions. ZC1 can phosphorylate SMAD1 at Thr-322. Whole-body knockouts of the ZC1 gene in mice are embryonic lethal, indicating its vital role in development, specifically in mesodermal and somite formation. Insertional mutagenesis studies in mice support a role for this protein kinase in mouse cancer oncogenesis. ZC1 is widely expressed in tumour cell lines and plays a significant role in promoting cell transformation and invasion. It acts as a pro-migratory kinase often associated with poor prognosis in cancers such as pancreatic, colorectal, and lung adenocarcinoma. This description may include information annotated by UniProt and/or Google AI.