Product Name:
HGK-pT187
Product Number:
ab-pk652
Target Full Name: Mitogen-activated protein kinase kinase kinase kinase 4
Target Alias: FLH21957; Hepatocyte progenitor kinase-like/germinal center kinase-like kinase; HGK; HPK/GCK-like kinase HGK; M4K4; MAP4K4; MAPK kinase kinase kinase 4; MAPK/ERK kinase kinase kinase 4; MEK kinase kinase 4; MEKKK 4; MEKKK4; Mitogen-activated protein kinase kinase kinase kinase 4; Nck interacting kinase; NIK; FLJ10410; FLJ20373; FLJ90111; KIAA0687; NIK; O95819; ENSG0000007105
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK653
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T187
Protein UniProt: O95819
Protein SigNET: O95819
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human HGK (ZC1) sequence peptide Cat. No.: PE-04ALN99
Antibody Immunogen Sequence: RRN(pT)FIG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R184 to G190; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues R184 to G190; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in serum-starved Jurkat cells treated with insulin for 5 minutes. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 135-150 kDa.
Antibody Specificity: High-very high
Related Product 1: HGK-pT187 blocking peptide
Related Product 2: HGK-NT pan-specific antibody (Cat. No.: AB-NK300-1)
Scientific Background: ZC1 (HGK, MAP4K4) is a protein-serine/threonine kinase of the STE group and STE20 family. It may play a role in the response to environmental stress and cytokines such as TNF-alpha, and appears to act upstream of the JUN N-terminal pathway. ZC1 can phosphorylate SMAD1 at T322. Insertional mutagenesis studies in mice support a role for this protein kinase in mouse cancer oncogenesis.
