Product Name:
GUK1-pY53
Product Number:
ab-pk651
Target Full Name: Guanylate kinase
Target Alias: GMK; GMP kinase; Guanylate kinase 1; KGUA
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK652
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y53
Protein UniProt: Q16774
Protein SigNET: Q16774
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human GUK1 sequence peptide Cat. No.: PE-04AMT99
Antibody Immunogen Sequence: GKD(pY)YFV(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues G50 to V56; In the first third of the Guanylate_kin domain. Is the major in vivo site of phosphorylation of GUK1.
Production Method: Corresponds to amino acid residues G50 to V56; In the first third of the Guanylate_kin domain. Is the major in vivo site of phosphorylation of GUK1.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity of a target-sized protein by Western blotting in MCF7 cells and sea star oocytes. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 23-28 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: Strong ~220, 50 and 35 kDa immuno-reactive proteins wre detected in sea star oocytes; a ~47 kDa cross-reactive proteins in HepG2 and MCF7 cells; and a strong 40 kDa cross-reactive protein in MCF7 cells.
Related Product 1: GUK1-pY53 blocking peptide
Scientific Background: GUK1 is a protein-serine/threonine kinase of the Atypical group and family. It is essential for recycling GMP and indirectly, cGMP.
