Product Name:
GSK3a-pY284+Y285
Product Number:
ab-pk649
Target Full Name: Glycogen synthase-serine kinase 3 alpha
Target Alias: DKFZp686D0638; Factor A; Glycogen synthase kinase 3 alpha; GSK-3 alpha; GSK3-alpha; Kinase GSK3-alpha
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK650
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y284+Y285
Protein UniProt: P49840
Protein SigNET: P49840
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human GSK3a sequence peptide Cat. No.: PE-04ABU95
Antibody Immunogen Sequence: SR(pY)(pY)RAP(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues S282 to P288; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues S282 to P288; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak detection immunoreactivity of a target-sized protein by Western blotting in A431 cells. Strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 50-54 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in A431 and Jurkat cells, although medium cross-reactive 37 and 26 kDa protein were observed in these cell lines.
Related Product 1: GSK3a-pY284+Y285 blocking peptide
Related Product 2: GSK3a-BKCD pan-specific antibody (Cat. No.: AB-NK069-3)
Related Product 3: GSK3a-pS278+pY279 phosphosite-specific antibody (Cat. No.: AB-PK647)
Related Product 4: GSK3a-pT19+pS21 phosphosite-specific antibody (Cat. No.: AB-PK648)
Related Product 5: GSK3a-pY279 phosphosite-specific antibody (Cat. No.: AB-PK649)
Related Product 6: GSK3Selectide - GSK3 protein kinase substrate peptide
Scientific Background: GSK3a is a protein-serine/threonine kinase of the CMGC group and GSK family. It is highly active following tyrosine phosphorylation within its activation T loop. Phosphorylation at Y279 increases phosphotransferase activity and promotes nuclear localization. Phosphorylation at S21 inhibits phosphotransferase activity. Through direct phosphorylation, it can inactivate glycogen synthase (for insulin regulation), CTNNB1/beta-catenin, APC, and AXIN1. GSK3 has been proposed as a tumour suppressor protein (TSP), since with loss of GSK3 there is an increase in WNT signalling, with a beta-catenin stabilization, leading to tumourigenesis. GSK3 has also been proposed to act as an oncoprotein (OP), as acute leukemia required GSK3 for cell proliferation. In most human cancers, it is up-regulated in expression about 1.6-times the rate observed for most protein kinases.
