Product Name:
GSK3a-pY279
Product Number:
ab-pk648
Target Full Name: Glycogen synthase-serine kinase 3 alpha
Target Alias: DKFZp686D0638; Factor A; Glycogen synthase kinase 3 alpha; GSK-3 alpha; GSK3-alpha; Kinase GSK3-alpha
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK649
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y279
Protein UniProt: P49840
Protein SigNET: P49840
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human GSK3a sequence peptide Cat. No.: PE-04ACX99
Antibody Immunogen Sequence: NVS(pY)ICS(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues N276 to S282; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues N276 to S282; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.3 / 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in HeLa cells following phenyarsine oxide and vanadate treatment for 30 min, and weak detection in MCF7 cells.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 50-54 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in A431, HeLa and MCF7 cells. In SDS-PAGE sample buffer extracted A431 cell lysates, it appears that there may be cross-reactivity with the EGF receptor.
Related Product 1: GSK3a-pY279 blocking peptide
Related Product 2: GSK3a-BKCD pan-specific antibody (Cat. No.: AB-NK069-3)
Related Product 3: GSK3a-pS278+pY279 phosphosite-specific antibody (Cat. No.: AB-PK647)
Related Product 4: GSK3a-pT19+pS21 phosphosite-specific antibody (Cat. No.: AB-PK648)
Related Product 5: GSK3a-pY284+Y285 phosphosite-specific antibody (Cat. No.: AB-PK650)
Related Product 6: GSK3Selectide - GSK3 protein kinase substrate peptide
Scientific Background: GSK3a is a protein-serine/threonine kinase of the CMGC group and GSK family. It is highly active following tyrosine phosphorylation within its activation T loop. Phosphorylation at Y279 increases phosphotransferase activity and promotes nuclear localization. Phosphorylation at S21 inhibits phosphotransferase activity. Through direct phosphorylation, it can inactivate glycogen synthase (for insulin regulation), CTNNB1/beta-catenin, APC, and AXIN1. GSK3 has been proposed as a tumour suppressor protein (TSP), since with loss of GSK3 there is an increase in WNT signalling, with a beta-catenin stabilization, leading to tumourigenesis. GSK3 has also been proposed to act as an oncoprotein (OP), as acute leukemia required GSK3 for cell proliferation. In most human cancers, it is up-regulated in expression about 1.6-times the rate observed for most protein kinases.
