Product Name:
Fused-pS159
Product Number:
ab-pk643
Target Full Name: Serine/threonine-protein kinase 36
Target Alias: FU; Fused serine/threonine kinase; KIAA1278; Serine/threonine kinase 36, fused; STK36; KIAA1278
Product Type Specific: Fused protein kinase phosphosite-specific antibody
Antibody Code: PK643
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S159
Protein UniProt: Q9NRP7
Protein SigNET: Fused
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Fused (STK36) sequence peptide Cat. No.: PE-04AMO99
Antibody Immunogen Sequence: VLT(pS)IKG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues V156 to G162; in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. S159 phosphorylation is predicted to be stimulatory for phosphotransferase activity. This is one of the minor in vivo phosphorylation sites in Fused (≥1 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity of a target-sized protein by Western blotting in MCF7 cells that are serum-starved. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 145-155 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in MCF7 and T98G cells or in sea star oocytes, but immunoreactive target band in MCF7 cells extends from 140 to 80 kDa.
Related Product 1: Fused (156-162) pS159 antibody blocking peptide (Cat. No.: PE-04AMO99)
Scientific Background: Fused (STK36) is a protein-serine/threonine kinase of the Other group and ULK family. It functions in the sonic hedgehog (Shh) signalling pathway. While its homologue in Drosophila is essential for the Hedgehog (Hh) pathway, vertebrate Fused has evolved a more specialized role in ciliogenesis rather than being essential for Hh signalling. However, it does contribute to the regulation of GLI transcription factors by mediating their nuclear localization in response to Hedgehog signaling. Fused regulates the activity of the transcription factors GLI1, GLI2, and GLI3 by opposing SUFU activity and stimulating nuclear translocation of the factors. In vertebrates, it is also recognized for its critical role in the assembly and function of the central pair apparatus in motile cilia (9+2 microtubule configuration). It regulates motile cilia structure and orientation in various tissues, including the respiratory epithelium and the brain ventricles. Fused directly interacts with several proteins involved in cilia structure, such as SPAG16 (a central pair protein) and KIF27 (a kinesin-like protein). It also forms a complex with ULK4 (a pseudokinase) and cooperates to regulate cilia function. In addition, Fused activity is necessary for post-natal development, potential through its role in ciliary function or regulation of cerebral spinal fluid homeostasis. Mutations in the Fused gene are associated with a form of Primary Ciliary Dyskinesia (PCD) (CILD46), a disease characterized by chronic respiratory infections, sinusitis, and infertility due to defective motile cilia. Dysfunction of motile cilia in the brain (ependymal cells) can lead to hydrocephalus (fluid buildup) due to poor cerebrospinal fluid flow. It has been implicated in certain cancers, such as prostate cancer, where it may promote epithelial-mesenchymal transition. This description may include information annotated by UniProt and/or Google AI.