Product Name:
Fused-pS159
Product Number:
ab-pk642
Target Full Name: Serine/threonine-protein kinase 36
Target Alias: FU; Fused serine/threonine kinase; KIAA1278; Serine/threonine kinase 36, fused; STK36; KIAA1278
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK643
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S159
Protein UniProt: Q9NRP7
Protein SigNET: Q9NRP7
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Fused (STK36) sequence peptide Cat. No.: PE-04AMO99
Antibody Immunogen Sequence: VLT(pS)IKG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues V156 to G162; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues V156 to G162; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity of a target-sized protein by Western blotting in MCF7 cells that are serum-starved. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 145-155 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in MCF7 and T98G cells or in sea star oocytes, but immunoreactive target band in MCF7 cells extends from 140 to 80 kDa.
Related Product 1: Fused-pS159 blocking peptide
Scientific Background: Fused (STK36) is a protein-serine/threonine kinase of the Other group and ULK family. It functions in the sonic hedgehog (Shh) signalling pathway. Fused regulates the activity of the transcription factors GLI1, GLI2, and GLI3 by opposing SUFU activity and stimulating nuclear translocation of the factors. Fused is also necessary for the assembly of the central pair apparatus in motile cilia. In addition, Fused activity is necessary for post-natal development, potential through its role in ciliary function or regulation of cerebral spinal fluid homeostasis.
