Product Name:
Frk-pY387
Product Number:
ab-pk641
Target Full Name: Fyn-related kinase; Tyrosine-protein kinase FRK
Target Alias: Nuclear tyrosine protein kinase RAK; PTK5; RAK; GTK; CCDS5103.1; ENSG00000111816
Product Type Specific: Frk protein kinase phosphosite-specific antibody
Antibody Code: PK641
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y387
Protein UniProt: P42685
Protein SigNET: Frk
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Frk sequence peptide Cat. No.: PE-04ARK80
Antibody Immunogen Sequence: EDI(pY)ESRH(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues E384 to H390; in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. Y387 phosphorylation is predicted to be stimulatory for phosphotransferase activity. This is one of the less major in vivo phosphorylation sites in Frk (≥68 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). Frk is known to be phosphorylated at this site in vitro by Frk.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very weak immunoreactivity of a target-sized protein by Western blotting in HeLa cells. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Sea urchin.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 50-58 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in HEK-293, Jurkat and T98G cells and sea star oocytes, except for a medium cross-reactive ~39 kDa protein in HeLa, HEK-293 and Jurkat cells and in sea star oocytes.
Related Product 1: Frk (384-390) pY387 antibody blocking peptide (Cat. No.: PE-04ARK80)
Related Product 2: Frk expression antibody (Cat. No.: AB-NK269-1)
Related Product 3: Frk expression antibody (Cat. No.: AB-NK269-2)
Related Product 4: Frk Y497 phosphorylation antibody (Cat. No.: AB-PK642)
Scientific Background: Frk (Fyn-related kinase) is a protein-tyrosine kinase of the TK group and Src family that is found in the cell nucleus. It is involved in positive regulation of PTEN by increasing its stability through phosphorylation of Tyr-336 and preventing it degradation, and negative regulation of cell proliferation. It may also have functions during G1 and S phase in the nucleus to suppress growth. While it is primarily found in the nucleus, it is also associated with the cytoplasm, extracellular vesicles, and specific granules. FRK is widely expressed in tissues, with high expression levels found in the kidney and duodenum. It may be a tumour suppressor protein (TSP) under certain circumstances. Cancer-related mutations in human tumours point to a loss of function of the protein kinase. Reduced expression has been observed in breast cancer, while mutations are linked to liver cancer (hepatocellular adenomas) and lung cancer. It may act as an oncogene in some cancers, like pancreatic and liver cancers, where its knockdown inhibits tumour growth, highlighting a functional paradox. Insertional mutagenesis studies in mice support a role for this protein kinase in mouse cancer oncogenesis. This description may include information annotated by UniProt and/or Google AI.