Product Name:
Frk-pY497
Product Number:
ab-pk641
Target Full Name: Fyn-related kinase; Tyrosine-protein kinase Frk
Target Alias: Nuclear tyrosine protein kinase RAK; PTK5; RAK; GTK; CCDS5103.1; ENSG00000111816
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK642
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y497
Protein UniProt: P42685
Protein SigNET: P42685
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Frk sequence peptide Cat. No.: PE-04AMN95
Antibody Immunogen Sequence: DSS(pY)SDA(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues D494 to A500; In the C-terminus of the kinase. One of the two major in vivo phosphorylation sites in Frk.
Production Method: Corresponds to amino acid residues D494 to A500; In the C-terminus of the kinase. One of the two major in vivo phosphorylation sites in Frk.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 50-58 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: No significant cross-reactive proteins detected in Jurkat and T98G cells, except phenylarsine oxide (PAO) treatment increases immunoreactivity of 15 kDa protein in Jurkat cells.
Related Product 1: Frk-pY497 blocking peptide
Related Product 2: Frk-ANT pan-specific antibody (Cat. No.: AB-NK269-1)
Related Product 3: Frk-NT pan-specific antibody (Cat. No.: AB-NK269-2)
Related Product 4: Frk-pY387 phosphosite-specific antibody (Cat. No.: AB-PK641)
Scientific Background: Frk is a protein-tyrosine kinase of the TK group and Src family that is found in the nucleus.. It is involved in positive regulation of PTEN by increasing its stability and preventing degradation and negative regulation of cell proliferation. It may also have functions during G1 and S phase in the nucleus to suppress growth. Frk may be a tumour suppressor protein (TSP). Cancer-related mutations in human tumours point to a loss of function of the protein kinase. nsertional mutagenesis studies in mice support a role for this protein kinase in mouse cancer oncogenesis.
