Product Name:
Fgr-pY412
Product Number:
ab-pk639
Target Full Name: Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene
Target Alias: C-Fgr; Fgr protein; Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene; Kinase Fgr; P55c-fgr; P55-Fgr; p58c-fgr; Proto-oncogene tyrosine-protein kinase Fgr; SRC2; FLJ43153; MGC75096; Q5TGY6; ENSG00000000938
Product Type Specific: Fgr protein kinase phosphosite-specific antibody
Antibody Code: PK639
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y412
Protein UniProt: P09769
Protein SigNET: Fgr
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Fgr sequence peptide Cat. No.: PE-04AMI90
Antibody Immunogen Sequence: DDE(pY)NPC(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues D409 to C415; in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. Y412 phosphorylation is predicted to be stimulatory for phosphotransferase activity. This is one of the major in vivo phosphorylation sites in Fgr (≥203 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Dog | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 55-63 kDa.
Antibody Specificity: Medium-High
Antibody Cross Reactivity: In MCF7 cells, relatively clean but high background at top and bottom of gel.
Related Product 1: Fgr (409-415) pY412 antibody blocking peptide (Cat. No.: PE-04AMI90)
Related Product 2: Fgr expression antibody (Cat. No.: AB-NK268-1)
Related Product 3: Fgr Y208+Y209 phosphorylation antibody (Cat. No.: AB-PK638)
Scientific Background: Fgr is a protein-tyrosine kinase of the TK group and Src family. It is a non-receptor kinase that transmits signals from cell surface receptors and regulates immune responses. In addition, it functions as positive regulator of cell migration and regulates cytoskeleton reorganization. Fgr appears to be an oncoprotein (OP). A Y523F mutation in Fgr is associated with strongly increased catalytic activity, which can be oncogenic and promote abnormal cell proliferation. Fgr oncogene mRNA was found to be induced in B lymphocytes by Epstein-Barr virus infection. This description may include information annotated by UniProt and/or Google AI.