Product Name:
FGFR1-pY653+pY654
Product Number:
ab-pk634
Target Full Name: Fibroblast growth factor receptor-tyrosine kinase 1; Basic fibroblast growth factor receptor 1
Target Alias: BFGFR; BFGF-R; CD331; CEK; C-fgr; FGFBR; FGFR-1; Fgr; FLT2; KAL2; Pfeiffer syndrome; HBGFR; KAL2; N-SAM; OGD; bFGF-R-1; H2; H3; H4; H5; ENSG00000077782
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK634
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y653+Y654
Protein UniProt: P11362
Protein SigNET: P11362
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human FGFR1 sequence peptide
Antibody Immunogen Sequence: HID(pY)(pY)KKT(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues H650 to T657; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues H650 to T657; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity of a target-sized protein by Western blotting in HeLa cells. Strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 100-110 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: Strong immunoreactivity on protein dot blots with recombinant human FGFR2 and weak with FGFR3 and FGFR4 on protein dot blots. The target phosphosites in FGFR1 are conserved in FGFR2. No significant cross-reactive proteins detected in A431, HeLa, MCF7 cells, but the top and bottom regions of gels had high backgrounds in MCF7 cells. No cross-reactive proteins were detected with sea star oocytes.
Related Product 1: FGFR1-1 pan-specific antibody (Cat. No.: AB-NK062-2)
Related Product 2: FGFR1-3 pan-specific antibody (Cat. No.: AB-NK062-3)
Scientific Background: FGFR1 (Flt2) is a protein-tyrosine kinase of the TK group and FGFR family. It is a fibroblast growth factor receptor, and is alternatively spliced generating multiple variants that are differentially expressed during embryogenesis and in the adult body. It is highly expressed and widely distributed in most tested human tissues. FGF receptors plays an important role in multiple biological processes, including mesoderm induction and patterning, cell growth and migration, organ formation and bone growth. FGFR1 is involved in cell proliferation, differentiation, migration, and embryonic development. FGFR1 is activated by basic-FGF, which induces dimerization and transautophosphorylation. Phosphorylation of Y653 and Y654 increases kinase activity. Phosphorylation of Y766 induces interaction of FGFR1 with PLCg1 and SHB, induces FRS2 phosphorylation, and activation of RAS and MAPK signalling, leading to cell proliferation. Phosphorylation of Y154 induces receptor internalization. It can phosphorylate GAB1, PLCG1, FRS2, and SHB. FGFR1 has been linked with the development of Pfeiffer Syndrome (PS), Kallmann Syndrome Type 2 (KAL2), Stem Cell Leukemia Lymphoma Syndrome (SCLL), breast carcinomas (ductal), lung bronchoalveolar carcinomas and lung large cell carcinomas.
