Product Name:
FAK-pY397
Product Number:
ab-pk627
Target Full Name: Focal adhesion protein-tyrosine kinase
Target Alias: FADK1; FAK1; pp125FAK; FRNK; Protein-tyrosine kinase 2; PTK2; CCDS6381.1; ENSG00000169398
Product Type Specific: FAK protein kinase phosphosite-specific antibody
Antibody Code: PK627
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y397
Protein UniProt: Q05397
Protein SigNET: FAK
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human FAK (PTK2) sequence peptide Cat. No.: PE-04AGQ99
Antibody Immunogen Sequence: TDD(pY)AEI(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues T394 to I400; in the region between the FERM_M and kinase catalytic domains. Y397 phosphorylation stimulates phosphotransferase activity and induces interaction with Src and other Src family kinases, Bmx, PIK3R1, RASA1 and SHC1, and this leads to phosphorylation at Y576, Y577 and additional tyrosine phosphosites. Phosphorylation inhibits interaction with PI3KR2. Phosphorylation regulates apoptosis, cell adhesion, cell differentiation, cell growth, cell motility, cytoskeletal reorganization, endocytosis, transcription, alters intracellular location, protein degradation and molecular associations. This is one of the major in vivo phosphorylation sites in FAK (≥485 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). FAK is known to be phosphorylated at this site in vitro by EphB2, FAK (PTK2), Fgr, Met, and Src.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity of a target-sized protein by Western blotting in HepG2 cells. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Mouse | Platypus | Chicken | Zebra fish | Honey bee.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 105-115 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: No significant cross-reactive proteins detected in MCF7 cells, but weak cross-reactivity with phospho-ERK1 in sea star oocytes.
Related Product 1: FAK (394-400) pY397 antibody blocking peptide (Cat. No.: PE-04AGQ99)
Related Product 2: FAKSubtide kinase substrate peptide (Cat. No.: PE-01BHJ95)
Related Product 3: FAK Y576+Y577 phosphorylation antibody (Cat. No.: AB-PK628)
Related Product 4: FAK Y577 phosphorylation antibody (Cat. No.: AB-PK629)
Related Product 6: FAK Y577 phosphorylation antibody (Cat. No.: AB-PK629)
Scientific Background: FAK (PTK2) is a protein-tyrosine kinase of the TK group and FAK family. It is a non-receptor kinase that regulates a variety of cell processes, including cell migration, adhesion, spreading, actin cytoskeletal reorganization, formation/disassembly of focal adhesions, progression through the cell cycle, cell proliferation, and apoptosis. In addition, the FAK protein is required for the proper development of the heart, skeleton, and nervous system. Phosphorylation of T397 increases phosphotransferse activity, induces interaction with FAK and SOCS1, and inhibits interaction with PIK3R2 and Src. Phosphorylation of Y576 and Y577 increases phosphotransferase activity. Phosphorylation of Y861 increases phosphotranserase activity and induces interaction with ITGB5 and p130 Cas. Phosphorylation of Y925 induces interaction with Grb2. Phosphorylation at S722 inhibits FAK binding to the adapter protein p130Cas. FAK appears to be an oncoprotein (OP). A role for FAK in cancer cell migration and motility has been demonstrated for several human cancer types. Knockdown of FAK expression in a human gastric adenocarinoma cell line resulted in the impairment of cancer cell spreading and elongation. In animal studies, deletion of the FAK gene in mice prevented papilloma formation and inhibited the progression of malignancy in pre-formed benign tumours, indicating an oncogenic role for the FAK protein. Additionally, FAK gene deletion was associated with a reduction in keratinocyte migration and increased keratinocyte cell death. Therefore, FAK was concluded to modulate the formation of benign tumours and their subsequent malignant conversion. In 39 hepatocellular carcinoma (HCC) cell lines analyzed for copy number expression, increased copy number was observed at the FAK locus and correlated strongly with expression level of the FAK protein as well as an increased tumour size (>5 cm). This description may include information annotated by UniProt and/or Google AI.