Product Name:
ERK5-pY221
Product Number:
ab-pk626
Target Full Name: Extracellular regulated protein-serine kinase 5 (Big MAP kinase 1 (BMK1))
Target Alias: BMK; BMK1; BMK1 kinase; ERK4; ERK-5; Extracellular-signal-regulated kinase 5; Kinase ERK5; MAPK7; PRKM7; ENSG00000166484
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK626
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y221
Protein UniProt: Q13164
Protein SigNET: Q13164
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human ERK5 (MAPK7) sequence peptide Cat. No.: PE-04AEI99
Antibody Immunogen Sequence: MTE(pY)VAT(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues M218 to T224; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues M218 to T224; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity of a target-sized protein by Western blotting in A431 cells. Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 88-115 kDa.
Antibody Specificity: Medium-High
Antibody Cross Reactivity: No significant cross-reactive proteins detected in T98G cells, but manycross-reactive proteins detected in A431 cells, including phospho-ERK and phospho-p38 MAP kinases.
Related Product 1: ERK5-pY221 blocking peptide
Related Product 2: ERK5-2 pan-specific antibody (Cat. No.: AB-NK206-4)
Related Product 3: ERK5-3 pan-specific antibody (Cat. No.: AB-NK206-5)
Related Product 4: ERK5-NT (ERK5-4) pan-specific antibody (Cat. No.: AB-NK206-2)
Related Product 5: ERK5-pT219+pY221 phosphosite-specific antibody (Cat. No.: AB-PK625)
Related Product 6: ERK5 -PNT (ERK5-5) pan-specific antibody (Cat. No.: AB-NK206-1)
Scientific Background: ERK5 (MAPK7) is a protein-serine/threonine kinase of the CMGC group and MAPK family. It is important in controlling a cell's proliferation, differentiation, and cell survival. Phosphorylation of T219 and Y221 by MEK5 (MAP2K5) increases the phosphotransferase activity of ERK5. MEK5 is activated in response to hyperosmolarity, hydrogen peroxide, and epidermal growth factor (EGF). ERK5 may be a tumour requiring protein (TRP). Although there has been no direct linkage of ERK5 to formation of carcinomas in humans in vivo, in vitro ERK5 over-expression has been shown to increase metastasis, and ERK5 inhibition has been shown to inhibit osteosarcoma cell motility. Upregulation of the MEK5 gene has been noted in breast, prostate, and liver cancer.
