Product Name:
ERK5-pT219+pY221
Product Number:
ab-pk625
Target Full Name: Extracellular regulated protein-serine kinase 5 (Big MAP kinase 1 (BMK1))
Target Alias: BMK; BMK1; BMK1 kinase; ERK4; ERK-5; Extracellular-signal-regulated kinase 5; Kinase ERK5; MAPK7; PRKM7; ENSG00000166484
Product Type Specific: ERK5 protein kinase phosphosite-specific antibody
Antibody Code: PK625
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T219+Y221
Protein UniProt: Q13164
Protein SigNET: ERK5
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human ERK5 (MAPK7) sequence peptide Cat. No.: PE-04AKG90
Antibody Immunogen Sequence: YFM(pT)E(pY)V(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues Y216 to V222; in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. T219 and Y221 phosphorylation stimulate phosphotransferase activity, and regulates apoptosis. The are major sites of phosphorylation of ERK5 in vivo with ≥33 and ≥981 mass spectrometry reports, respectively, in PhosphoSitePlus. ERK5 is known to be phosphorylated in vitro at T219 by MEK5 (MAP2K5), and at Y221 by MEK5 (MAP2K5).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity of a target-sized protein by Western blotting in maturing sea star oocytes and A431 cells. Weak detection of ERK5 in HEK-293, HeLa, HepG2, and MCF7. Strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus Macaque | Rat | Mouse | Zebra fish.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 88-115 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant human ERK2. No significant cross-reactive proteins detected in A431, HEK-293, HeLa, HepG2 and MCF7 cells and sea star oocytes, except for strong cross-reactivity with phospho-ERK1 and phospho-ERK2, and also in sea star oocytes 3 proteins with molecular masses greater than 120 kDa.
Related Product 1: ERK5 (216-222) pT219+pY221 antibody blocking peptide (Cat. No.: PE-04AKG90)
Related Product 2: ERK5 expression antibody (Cat. No.: AB-NK206-1)
Related Product 3: ERK5 expression antibody (Cat. No.: AB-NK206-4)
Related Product 4: ERK5 expression antibody (Cat. No.: AB-NK206-5)
Related Product 5: ERK5 Y221 phosphorylation antibody (Cat. No.: AB-PK626)
Related Product 6: ERK5 T219 phosphorylation antibody (Cat. No.: AB-PK968)
Scientific Background: ERK5 (MAPK7) is a 98 kDa protein-serine/threonine kinase of the CMGC group and MAPK family. It is important in controlling a cell's proliferation, differentiation, and. survival, and angiogenesis. Phosphorylation of T219 and Y221 in a 'TEY" motif by MEK5 (MAP2K5) increases the phosphotransferase activity of ERK5. MEK5 is activated in response to hyperosmolarity, hydrogen peroxide, and epidermal growth factor (EGF). While ERK5 exists in both the cytoplasm and nucleus, its activation often induces translocation to the nucleus, where it activates transcription factors such as MEF2C and c-Myc. MEF2A/C/D, connexin-43 and SGK1 are also substrates for ERK5. It is highly expressed in heart, skeletal muscle, vascular endothelium, and the nervous system. ERK5 may be a tumour requiring protein (TRP). Although there has been no direct linkage of ERK5 to formation of carcinomas in humans in vivo, in vitro, ERK5 over-expression has been shown to increase metastasis, and ERK5 inhibition has been shown to inhibit osteosarcoma cell motility. Upregulation of the MEK5 gene has been noted in breast, prostate, and liver cancer. This description may include information annotated by UniProt and/or Google AI.