Product Name:
ERK4-pS186
Product Number:
ab-pk624
Target Full Name: Extracellular regulated protein-serine kinase 4
Target Alias: MAP kinase isoform p63; MAPK4; MK04; P63-MAPK; PRKM4; ERK-3 related; extracellular signal-regulated kinase 4; ENSG00000141639
Product Type Specific: ERK4 protein kinase phosphosite-specific antibody
Antibody Code: PK624
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S186
Protein UniProt: P31152
Protein SigNET: ERK4
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human ERK4 (MAPK4) sequence peptide
Antibody Immunogen Sequence: GYL(pS)EGLV(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues G183 to V190. S186 phosphorylation stimulates interaction with MAPKAPK5 and probably phosphorylation stimulates phosphotransferase activity. This phosphosite is located in the kinase activation loop between catalytic subdomains VII and VIII. This is the major in vivo phosphorylation site in ERK4 (≥16 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus Macaque | Dog | Rat | Mouse | Chicken | Zebra fish.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 55-65 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in HEK-293 and Jurkat cells, and seastar oocytes, except for phospho-ERK1.
Related Product 1: ERK1 T202+Y204 phosphorylation antibody (Cat. No.: AB-PK621)
Related Product 2: ERK1 Y204 phosphorylation antibody (Cat. No.: AB-PK864)
Related Product 3: ERK2 T185+Y187 phosphorylation antibody (Cat. No.: AB-PK622)
Related Product 4: ERK3 S189 phosphorylation antibody (Cat. No.: AB-PK623)
Related Product 5: ERK5 T219 phosphorylation antibody (Cat. No.: AB-PK968)
Related Product 6: ERK5-pY221 phosphosite-specific antibody (Cat. No.: AB-PK626)
Related Product 7: ERK5 T219+Y221 phosphorylation antibody (Cat. No.: AB-PK625)
Scientific Background: ERK4 (MAPK4) is a protein-serine/threonine kinase of the CMGC group and MAPK family. It functions to phosphorylate microtubule-associated protein 2 (MAP2) and MAPKAPK5, which may have a role in promoting the entrance of cells into the cell cycle. ERK3 and ERK4 share high sequence homology and are hypothesized to be paralogues. In animal studies, mice lacking ERK3 show a phenotype that closely resembles human pulmonary immaturity, along with reduced fetal growth potential and significantly lower neonatal survival rates. Additional loss of ERK4 in mice lacking ERK3 does not result in the worsening of symptoms of either the neonatal growth deficiency or the pulmonary immaturity phenotype, indicating that ERK4 is dispensible for mouse embryonic development with regards to pulmonary immaturity and growth potential. Therefore, reduced ERK4 protein expression may contribute to the pathogenesis of pulmonary immaturity, potentially due to a reduced entrance of cells into the cell cycle during pulmonary development. This description may include information annotated by UniProt and/or Google AI.