Product Name:
ERK4-pS186
Product Number:
ab-pk624
Target Full Name: Extracellular regulated protein-serine kinase 4
Target Alias: MAP kinase isoform p63; MAPK4; MK04; P63-MAPK; PRKM4; ERK-3 related; extracellular signal-regulated kinase 4; ENSG00000141639
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK624
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S186
Protein UniProt: P31152
Protein SigNET: P31152
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human ERK4 (MAPK4) sequence peptide
Antibody Immunogen Sequence: GYL(pS)EGLV(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues G183 to V190; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues G183 to V190; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 55-65 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in HEK-293 and Jurkat cells, and seastar oocytes, except for phospho-ERK1.
Scientific Background: ERK4 (MAPK4) is a protein-serine/threonine kinase of the CMGC group and MAPK family. It functions to phosphorylate microtubule-associated protein 2 (MAP2) and MAPKAPK5, which may have a role in promoting the entrance of cells into the cell cycle. ERK3 and ERK4 share high sequence homology and are hypothesized to be paralogues. In animal studies, mice lacking ERK3 show a phenotype that closely resembles human pulmonary immaturity, along with reduced fetal growth potential and significantly lower neonatal survival rates. Additional loss of ERK4 in mice lacking ERK3 does not result in the worsening of symptoms of either the neonatal growth deficiency or the pulmonary immaturity phenotype, indicating that ERK4 is dispensible for mouse embryonic development with regards to pulmonary immaturity and growth potential. Therefore, reduced ERK4 protein expression may contribute to the pathogenesis of pulmonary immaturity, potentially due to a reduced entrance of cells into the cell cycle during pulmonary development.
