Product Name:
ERK3-pS189
Product Number:
ab-pk623
Target Full Name: Extracellular regulated protein-serine kinase 3
Target Alias: MAPK6; Mitogen-activated protein kinase 6; MK06; P55-MAPK; P97-MAPK; PRKM4; PRKM6; DKFZp686F03189; HsT17250; CCDS10147.1; ENSG00000069956
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK623
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S189
Protein UniProt: Q16659
Protein SigNET: Q16659
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human ERK3 (MAPK6) sequence peptide Cat. No.: PE-04AKF90
Antibody Immunogen Sequence: GHL(pS)EGL(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues G186 to L192; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues G186 to L192; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very weak immunoreactivity of a target-sized protein by Western blotting in MCF7 cells.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 95-100 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in A431 cells, except a 75 kDa cross-reactive protein can be observed in both celll lnes.
Related Product 1: ERK3-pS189 blocking peptide
Scientific Background: ERK3 (MAPK6) is a protein-serine/threonine kinase of the CMGC group and MAPK family. ERK3 is highly expressed in the skeletal muscle and the brain. It is located in nucleus and has been shown to be activating in fibroblasts with serum or phorbol esters treatments. Phosphorylation at S189 increases its phosphotransferase activity. Overexpression of ERK3 in fibroblasts inhibits S-phase entry. It may act as a negative regulator of cell proliferation. It is downregulated in different tumour types. However regulation and function of ERK3 remains to be fully elucidated. It phosphorylates MAP2.
