Product Name:
ERK3-pS189
Product Number:
ab-pk623
Target Full Name: Extracellular regulated protein-serine kinase 3
Target Alias: MAPK6; Mitogen-activated protein kinase 6; MK06; P55-MAPK; P97-MAPK; PRKM4; PRKM6; DKFZp686F03189; HsT17250; CCDS10147.1; ENSG00000069956
Product Type Specific: ERK3 protein kinase phosphosite-specific antibody
Antibody Code: PK623
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S189
Protein UniProt: Q16659
Protein SigNET: ERK3
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human ERK3 (MAPK6) sequence peptide Cat. No.: PE-04AKF90
Antibody Immunogen Sequence: GHL(pS)EGL(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues G186 to L192; in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. S189 phosphorylation stimulates phosphotransferase activity and induces interaction with MAPKAPK5. This is one of the major in vivo phosphorylation sites in ERK3 (≥S189 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). ERK3 is known to be phosphorylated at this site in vitro by ERK3, PAK1, PAK2, and PAK3.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very weak immunoreactivity of a target-sized protein by Western blotting in MCF7 cells.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus Macaque | Dog | Rat | Mouse | Chicken | Zebra fish.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 95-100 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in A431 cells, except a 75 kDa cross-reactive protein can be observed in both celll lnes.
Related Product 1: ERK3 (186-192) pS189 antibody blocking peptide (Cat. No.: PE-04AKF90)
Related Product 2: ERK1 T202+Y204 phosphorylation antibody (Cat. No.: AB-PK621)
Related Product 3: ERK1 Y204 phosphorylation antibody (Cat. No.: AB-PK864)
Related Product 4: ERK2 T185+Y187 phosphorylation antibody (Cat. No.: AB-PK622)
Related Product 5: ERK4 S186 phosphorylation antibody (Cat. No.: AB-PK624)
Related Product 6: ERK5 T219+Y221 phosphorylation antibody (Cat. No.: AB-PK625)
Related Product 7: ERK5 T219 phosphorylation antibody (Cat. No.: AB-PK968)
Related Product 8: ERK5 Y221 phosphorylation antibody (Cat. No.: AB-PK626)
Related Product 9: ERK5 T219 phosphorylation antibody (Cat. No.: AB-PK968)
Scientific Background: ERK3 (MAPK6) is a protein-serine/threonine kinase of the CMGC group and MAPK family. It is 62 kDa protein with a long C-terminal extension, although it can also be found in a truncated 45 kDaform. It regulates neuronal morphogenesis, inflammation, metabolism, and epithelial cell structure. ERK3 is highly expressed in the skeletal muscle and the brain. It is located in nucleus and has been shown to be activating in fibroblasts with serum or phorbol esters treatments. Phosphorylation at S189 increases its phosphotransferase activity. This resides in a SEG motif rather than the TXY double phosphorylation motif ound in other MAPK. ERK3 acts as a key regulator and activator of MAPK-activated protein kinase 5 (MK5, also known as PRAK), forming a stable complex. Overexpression of ERK3 in fibroblasts inhibits S-phase entry. It may act as a negative regulator of cell proliferation. It is downregulated in different tumour types. However regulation and function of ERK3 remains to be fully elucidated. It phosphorylates MAP2. This description may include information annotated by UniProt and/or Google AI.