Product Name:
ErbB3-pY1289
Product Number:
ab-pk616
Target Full Name: ErbB3 (HER3) receptor-tyrosine kinase
Target Alias: C-erbB3; ERB3; HER3; Kinase ErbB3; LCCS2; Receptor protein-tyrosine kinase erbB-3 precursor; Tyrosine kinase-type cell surface receptor HER3; V-erb-b2 erythroblastic leukemia viral oncogene 3; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; MGC88033; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3; ENSG00000065361; O75810; O75811; O75812; O75813; Q9BUD7; Q9NNX2; Q9NNX3
Product Type Specific: ErbB3 protein kinase phosphosite-specific antibody
Antibody Code: PK616
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y1289
Protein UniProt: P21860
Protein SigNET: ErbB3
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human ErbB3 (HER3) sequence peptide Cat. No.: PE-04AGZ95
Antibody Immunogen Sequence: EQG(pY)EEM(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues E1286 to M1292; in the cytoplasmic region of ErbB3 near the C-terminus after the kinase catalytic domain. Y1289 phosphoryation induces interaction with PIK3R2 and regulates carcinogenesis, and cell growth. This is one of the major in vivo phosphorylation sites in ErbB3 (≥134 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). ErbB3 is known to be phosphorylated at this site in vitro by ROR1.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in A431 cells.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse | Platypus.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 160-200 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: In Jurkat cells, phenylarsine oxide (PAO) increases detection of a 15 kDa cross-reactive protein.
Related Product 1: ErbB3 (1286-1292) pY1289 antibody blocking peptide (Cat. No.: PE-04AGZ95)
Related Product 2: ErbB3 expression antibody (Cat. No.: AB-NK231-2)
Related Product 3: ErbB3 expression antibody (Cat. No.: AB-NK231-3)
Related Product 4: ErbB3 expression antibody (Cat. No.: AB-NK231-4)
Related Product 5: ErbB3 Y1307 phosphorylation antibody (Cat. No.: AB-PK617)
Related Product 6: ErbB3 Y1328 phosphorylation antibody (Cat. No.: AB-PK618)
Related Product 7: ErbB4 expression antibody (Cat. No.: AB-NK235-1)
Related Product 8: ErbB4 expression antibody (Cat. No.: AB-NK235-2)
Related Product 9: ErbB4 expression antibody (Cat. No.: AB-NK235-3)
Related Product 10: ErbB4 Y733 phosphorylation antibody (Cat. No.: AB-PK619)
Scientific Background: ErbB3 (HER3) is a protein-tyrosine kinase of the TK group and EGFR family. It is a receptor kinase that bind and be activated by NTAK and neuregulins. It has been proposed to be deficient in phosphotransferase activity. While the kinase domain appararently lacks activity but heterodimerizes with other EGFRs permits transduction of growth signals. Phosphorylation of Tyr-1222 and Tyr-1260 induces interaction with PIK3C and PIK3R1. ErbB3 appears to be up-regulated in many cancer, including medulloblastomas, verrucous carcinomas, childhood medulloblastomas, carotid body tumours (CBT), and gestational trophoblastic tumours (GTD). This description may include information annotated by UniProt and/or Google AI.